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Updated: Jul 5, 2026
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Phenotypic Analysis and Isolation of Murine Hematopoietic Stem Cells and Lineage-committed Progenitors
Published on: July 8, 2012
Isolation of murine hematopoietic stem cells and progenitor cells
Hideo Ema1, Yohei Morita, Hiromitsu Nakauchi
1The University of Tokyo, Tokyo, Japan.
Current Protocols in Immunology
|April 25, 2008
Abstract:
Protocols for the purification of CD34-KSL cells, CD34+KSL cells, SP cells, and Tip-SP CD34-KSL cells are described. Mouse hematopoietic stem cells are highly enriched in the CD34-KSL and SP fractions. These two populations overlap one another. A nearly homogenous population of HSCs can be obtained by isolating Tip-SP CD34-KSL cells. One of the earliest progenitor cell populations is CD34+KSL cells.
