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Updated: Jul 5, 2026
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Conformational Evaluation of HIV-1 Trimeric Envelope Glycoproteins Using a Cell-based ELISA Assay
Published on: September 14, 2014
Detection assays for HIV proteins
Mario A Ostrowski1, Tae-Wook Chun2, Bruce Cheseboro2
1University of Toronto, Toronto, Canada.
Current Protocols in Immunology
|April 25, 2008
Summary
This study details four methods for detecting human immunodeficiency virus (HIV) proteins. Assays include ELISA, immunoblotting, immunofluorescence, and a functional test for reverse transcriptase activity, aiding in viral production assessment.
Area of Science:
- Virology
- Immunology
- Biochemistry
Background:
- Accurate detection of human immunodeficiency virus (HIV) proteins is crucial for diagnosis and monitoring.
- Existing methods require updated protocols for comprehensive analysis.
Purpose of the Study:
- To describe four distinct assays for the detection of HIV proteins.
- To provide methods for both qualitative and quantitative assessment of viral markers.
Main Methods:
- Quantitative enzyme-linked immunosorbent assay (ELISA) for protein detection.
- Quantitative immunoblotting assay for specific protein identification.
- Qualitative immunofluorescence assay for protein localization.
- Functional assay measuring reverse transcriptase activity to indicate viral production.
Main Results:
- The unit outlines four validated assays for HIV protein detection.
- These methods cover a range of detection sensitivities and specificities.
- The functional assay provides a direct measure of active viral replication.
Conclusions:
- The described assays offer a comprehensive toolkit for HIV research and diagnostics.
- These methods facilitate the assessment of viral presence and activity.
- This updated unit enhances the capabilities for studying HIV infection.
