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Establishment of Epstein-Barr Virus Growth-transformed Lymphoblastoid Cell Lines
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Establishment of Epstein-Barr Virus Growth-transformed Lymphoblastoid Cell Lines

Published on: November 8, 2011

Generation of Epstein-Barr Virus (EBV)-immortalized B cell lines

Giovanna Tosato1, Jeffrey I Cohen2

  • 1National Cancer Institute, Bethesda, Maryland.

Current Protocols in Immunology
|April 25, 2008
PubMed

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Cell Lines01:16

Cell Lines

A cell line is a population of cells grown in vitro that can be subcultured over several generations. Normal cells cease to divide after a certain number of cell divisions, a process known as replicative senescence. This number, called the Hayflick limit, was conceptualized by Leonard Hayflick in 1961 when he observed that fetal cells grown in culture could only divide 40-60 times. This limit is due to the shortening of the telomeres during each round of cell division, preventing cell division...