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Updated: Jul 5, 2026
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Gene Expression Profiling of Infecting Microbes Using a Digital Bar-coding Platform
Published on: January 13, 2016
[Serial analysis of gene expression in parasitological research]
Qiao-Li Li1, Zhi-Ming Zhang, Zhong-Dong Qiao
1College of Life Science & Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China.
Summary
Serial analysis of gene expression (SAGE) quantifies gene activity by sequencing short RNA tags. This method aids in understanding cellular conditions and gene expression profiles, particularly in parasite biology.
Area of Science:
- Molecular Biology
- Genomics
- Bioinformatics
Context:
- High-throughput gene expression analysis is crucial for understanding cellular function.
- Serial analysis of gene expression (SAGE) provides a quantitative method for global gene expression profiling.
- Identifying specific genes related to cellular conditions requires comparative expression analysis.
Purpose:
- To provide a comprehensive overview of the Serial analysis of gene expression (SAGE) technique.
- To detail the protocol, methodological advancements, and applications of SAGE.
- To highlight the utility of SAGE in parasite biology research.
Summary:
- Serial analysis of gene expression (SAGE) is a high-throughput technique for quantitative, global gene expression analysis in eukaryotes.
- It relies on sequencing short tags (10-14 bp) derived from mRNA to identify and quantify transcripts.
- Bioinformatics and statistical analysis of these tags reveal gene abundance and facilitate comparisons between different cellular conditions.
Impact:
- SAGE enables the identification of gene expression profiles in various cell types and tissues.
- It aids in discovering condition-specific genes by comparing expression patterns.
- The review emphasizes SAGE's significant applications in advancing parasite biology research.
