Related Experiment Video

Updated: Jul 5, 2026

Determination of Protein Expression Level in Cultured Cells by Immunocytochemistry on Paraffin-embedded Cell Blocks
09:06

Determination of Protein Expression Level in Cultured Cells by Immunocytochemistry on Paraffin-embedded Cell Blocks

Published on: May 20, 2018

Expression of calcyclin-binding protein/Siah-1 interacting protein in normal and malignant human tissues: an

Huihong Zhai1, Yongquan Shi, Haifeng Jin

  • 1State Key Laboratory of Cancer Biology, Institute of Digestive Diseases, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China.

The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society
|April 30, 2008
PubMed

Insights

Calcyclin-binding protein (CacyBP)/Siah-1 interacting protein (SIP) is highly expressed in various cancers. This study maps CacyBP/SIP expression in normal and tumor tissues, revealing its ubiquitous presence in tumors and potential role in tumorigenesis.

Area of Science:

  • Molecular Biology
  • Cancer Research
  • Proteomics

Background:

  • Calcyclin-binding protein (CacyBP)/Siah-1 interacting protein (SIP) is involved in ubiquitin-mediated proteolysis and has been linked to beta-catenin degradation.
  • Previous research indicated CacyBP/SIP modulates multidrug resistance in gastric cancer and is upregulated in these tissues.

Purpose of the Study:

  • To comprehensively analyze the protein expression profile of CacyBP/SIP across a wide spectrum of human normal tissues and carcinomas.
  • To establish a baseline understanding of CacyBP/SIP expression patterns for future functional studies in tumorigenesis.

Main Methods:

  • Immunohistochemistry staining using a newly developed anti-CacyBP/SIP monoclonal antibody.
  • Analysis of CacyBP/SIP protein localization (cytoplasm/nucleus) and expression levels in diverse human tissues and cancer types.

Main Results:

  • CacyBP/SIP exhibited positive staining in brain, heart, lymph node, and esophagus, with weak staining in the rectum and kidney; it was undetectable in most other normal tissues.
  • In contrast, CacyBP/SIP was ubiquitously detected in all analyzed tumor tissues.
  • Notably high expression levels of CacyBP/SIP were observed in nasopharyngeal carcinoma, osteogenic sarcoma, and pancreatic cancer.

Conclusions:

  • This study presents the first extensive analysis of CacyBP/SIP expression in human normal and tumor tissues.
  • The findings highlight CacyBP/SIP as a potential pan-cancer marker with significantly elevated expression in numerous malignancies.
  • This expression data provides a crucial reference for future research into the functional roles of CacyBP/SIP in cancer development.

More Related Videos

Visualizing the Calcitonin Gene-Related Peptide Immunoreactive Innervation of the Rat Cranial Dura Mater with Immunofluorescence and Neural Tracing07:28

Visualizing the Calcitonin Gene-Related Peptide Immunoreactive Innervation of the Rat Cranial Dura Mater with Immunofluorescence and Neural Tracing

Published on: January 6, 2021

Related Concept Videos

Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the daughter...
Synthesis and Functions of Calcitonin00:51

Synthesis and Functions of Calcitonin

Calcitonin, a vital polypeptide hormone, regulates calcium levels within body fluids. It is released by the parafollicular cells, also known as C cells, situated in the follicular epithelium of the thyroid gland. Calcitonin responds to fluctuations in blood calcium levels and the influence of gastrointestinal hormones like gastrin and cholecystokinin.
The exact mechanisms by which calcitonin operates in calcium homeostasis remain elusive, but its significance is evident in several vital...