Preparation, characterization and evaluation of Marsupsin-phospholipid complex
Mukesh S Sikarwar1, Shalini Sharma, Amit K Jain
1Department of Pharmacognosy and Phytochemistry, B. R. Nahata College of Pharmacy, Mhow-Neemuch Road, Mandsaur 458001, India. mukeshsikarwar@gmail.com
AAPS Pharmscitech
|May 1, 2008
Summary
This study developed a Marsuppin-phospholipid complex to enhance marsuppin bioavailability. The new formulation significantly improved oral absorption and biological response compared to the standard form.
Area of Science:
- Pharmacology
- Drug Delivery Systems
- Materials Science
Background:
- Marsuppin's bioavailability is limited, hindering its therapeutic efficacy.
- Phospholipid complexation is a strategy to improve drug absorption and delivery.
Purpose of the Study:
- To formulate and characterize a Marsuppin-phospholipid complex (M-P Complex).
- To evaluate the enhanced bioavailability and biological response of the M-P Complex.
Main Methods:
- Mechanical dispersion method for M-P Complex formulation.
- Characterization using Transmission Electron Microscopy (TEM), IR, 1H-NMR, and RP-HPLC.
- In vitro release studies and in vivo pharmacokinetic evaluation in albino rabbits.
Main Results:
- M-P Complex formation confirmed by analytical techniques.
- TEM revealed M-P Complex particle size in the range of 0.05-0.5 micrometers.
- Entrapment efficiency was 44% with a first-order release profile.
- Significantly increased oral bioavailability and improved C(max) (3.02 mg/ml) and T(max) (10.2 h) observed.
Conclusions:
- Complexing marsuppin with phospholipids effectively enhances its oral bioavailability.
- The M-P Complex offers improved biological response compared to free marsuppin.
- This formulation presents a promising approach for marsuppin delivery.
