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Color development time of the Lowry protein assay

Christopher M Pomory1

  • 1Department of Biology, University of West Florida, Pensacola, FL 32514, USA. cpomory@uwf.edu

Analytical Biochemistry
|May 2, 2008
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Western Blotting01:15

Western Blotting

Western blotting is an analytical technique for protein identification. It has various applications in immunology and medicine, including detecting diseases like bovine spongiform encephalopathy, mad cow disease, and human and feline immunodeficiency virus from biological samples.
The technique begins with separating proteins from the sample using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), followed by protein transfer, immunoblotting, and finally, protein detection.