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Method development for HILIC assays.

Bieke Dejaegher1, Debby Mangelings, Yvan Vander Heyden

  • 1Department of Analytical Chemistry and Pharmaceutical Technology, Pharmaceutical Institute, Vrije Universiteit Brussel - VUB, Brussels, Belgium.

Journal of Separation Science
|May 8, 2008
PubMed
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This review highlights method development for hydrophilic interaction LC (HILIC), a technique for separating polar compounds. It details univariate approaches used in literature and suggests strategies for multivariate development.

Area of Science:

  • Analytical Chemistry
  • Chromatography

Background:

  • Hydrophilic interaction LC (HILIC) is a chromatographic technique utilizing aqueous-organic mobile phases and hydrophilic stationary phases.
  • It is primarily employed for the separation of polar and hydrophilic compounds.

Purpose of the Study:

  • To review and highlight method development strategies for HILIC.
  • To discuss analytes, factors, responses, and conditions for HILIC methods found in the literature.
  • To present a potential strategy for multivariate HILIC method development due to limited publications.

Main Methods:

  • Review of existing literature on HILIC method development.
  • Analysis of univariate method development approaches, examining factors, responses, and conditions.
  • Exploration of potential multivariate method development strategies using experimental designs.

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Main Results:

  • All published HILIC methods reviewed were developed using a univariate, one-at-a-time approach.
  • Details on analytes, factors, responses, and experimental conditions for these univariate methods are discussed.
  • A strategy for multivariate HILIC method development is proposed, addressing the lack of published examples.

Conclusions:

  • Univariate method development has been the standard for HILIC, but multivariate approaches offer potential for optimization.
  • Further research and publications are needed to explore and validate multivariate strategies for HILIC method development.