Related Experiment Video
Updated: Jul 5, 2026
A Convenient and General Expression Platform for the Production of Secreted Proteins from Human Cells
Published on: July 31, 2012
[Construction of the eukaryotic expression vector pEGFP-claudin-1 and its expression in 293T cells]
1Department of Infectious Diseases, Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, China. weixinxp@gmail.com
Aim:
To construct the eukaryotic expression vector pEGFP-claudin-1 and to make it express expressed in 293T cells.
Methods:
Claudin-1 ORF was amplified by reverse transcription polymerase chain reaction (RT-PCR). The eukaryotic expression vector pEGFP-claudin-1 was constructed by introducing claudin-1 DNA fragment into the sites of Xho I and BamH I of pEGFP-C3 vector. The plasmid was transfected into 293T cells with lipofectamine. The expressed EGFP was observed by fluorescent microscope. The EGFP-claudin-1 was detected by Western blot.
Results:
The eukaryotic expression vector pEGFP-claudin-1 was constructed successfully. The expression of EGFP-claudin-1 was observed in the membrane of transfected cells by fluorescent microscope and a 49 kD protein was detected by Western blot.
Conclusion:
The recombinant expression vector pEGFP-claudin-1 has been successfully constructed and claudin-1 gene can be expressed in 293T cells. This study lays a foundation for further research into the function of claudin-1.
More Related Videos
10:18Generation of Plasmid Vectors Expressing FLAG-tagged Proteins Under the Regulation of Human Elongation Factor-1α Promoter Using Gibson Assembly
Published on: February 9, 2015
12:59Optimized Transfection Strategy for Expression and Electrophysiological Recording of Recombinant Voltage-Gated Ion Channels in HEK-293T Cells
Published on: January 19, 2011