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Updated: Jul 5, 2026
Multiplex Detection of Bacteria in Complex Clinical and Environmental Samples using Oligonucleotide-coupled Fluorescent Microspheres
Published on: October 23, 2011
[Construction for detecting of human papillomaviruses types by using a multiplexed Luminex assay]
Qian-li Dang1, Chao Chen, Peng DU
1Department of Dermatology, Shannxi Provincial People's Hospital, Xi'an 710068, China. dangql@yahoo.com.cn
Aim:
To develop multiplex human papillomavirus genotyping, a quick and sensitive high-throughput procedure for the identification of multiple HPV genotypes by using a multiplexed Luminex array.
Methods:
Standard plasmids containing HPV genomic DNA were developed a standard multiplexed suspension array for detection 13 HPV types on Luminex analysis platform. the primer and probes were selected, HPV type-specific oligonucleotide probes were coupled to fluorescence-labeled polystyrene beads.
Results:
The one or two of mixed HPV standard plasmids were identified HPV type by using 13 HPV genetypes multiplexed suspension array, different HPV types can be correctly detected simultaneously. The positives of HPV clinical samples were detected by using this method, and the genetypes of samples by detecting were the same results with using BLAST in NCBI.
Conclusion:
The proposed method allowed a high through-out, special, simple, rapid and economical identification of HPV DNA genotypes, It is expected to be an extremely useful tool in the identification of HPV genetypes for a lot of clinical samples.
