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Updated: Jul 5, 2026
Optimized Interferon-gamma ELISpot Assay to Measure T Cell Responses in the Guinea Pig Model after Vaccination
Published on: January 20, 2019
[Development of sandwich ELISA for equine interferon-gamma detection]
Yu Bai1, Tie-gang Tong, Wei-jun Zhang
1National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute CAAS, Harbin 150001, China. baiyu106@163.com
Aim:
To develop a quantitative ELISA by measuring interferon (IFN-gamma) of equine lymphocytes.
Methods:
Sandwich ELISA for equine IFN-gamma was developed using mAb A5 as a capture antibody and biotinylated mAb SB10 as a detection antibody.
Results:
The detection limit of the sandwich ELISA for equine IFN-gamma was 1 microg/L and did not show cross-reactivity with recombinant equine IL-18. Equine IFN-gamma was detected by ELISA in culture medium of the peripheral blood mononuclear cells (PBMCs) stimulated with ConA or PMA/Ionomycin.
Conclusion:
This method can be used to help understand the role of this cytokine in various equine diseases and develop specific cell-mediated immunity assay.
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