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Increased TNF expression in CD43++ murine blood monocytes
Bernard Burke1, Rasheedah Ahmad, Karl J Staples
1Department of Infection, Immunity and Inflammation, University of Leicester, Leicester, UK.
Immunology Letters
|May 13, 2008
Summary
Researchers identified a specific mouse monocyte subset (CD43++ GR-1-) that acts like human pro-inflammatory monocytes. This subset is depleted during infection and shows higher TNF production, crucial for understanding immune responses.
Area of Science:
- Immunology
- Cell Biology
Background:
- Monocyte heterogeneity is well-studied in humans, but mouse models are emerging.
- The MacGreen mouse model expresses green fluorescent protein (GFP) under the M-CSF receptor promoter.
Purpose of the Study:
- To characterize mouse blood monocyte subpopulations using the MacGreen model.
- To investigate the role of these subpopulations in response to inflammatory stimuli and infection.
Main Methods:
- Utilized the MacGreen mouse model with GFP expression.
- Excluded granulocytes using Ly6G staining.
- Subdivided monocytes into CD43+ GR-1+ and CD43++ GR-1(-) populations.
- Analyzed monocyte depletion after LPS injection and S. pneumoniae infection.
- Assessed TNF production via in vitro stimulation, Brefeldin A treatment, intracellular staining, and multicolor flow cytometry.
Main Results:
- Identified GFP+ Ly6G- blood monocytes (average 246 cells/µl).
- Subdivided monocytes into CD43+ GR-1+ and CD43++ GR-1(-) subsets (CD43++ GR-1(-) comprising ~60%).
- Observed depletion of both subsets after LPS and S. pneumoniae infection, with preferential reduction of CD43++ GR-1(-) monocytes during pneumococcal infection.
- Demonstrated significantly higher TNF protein production in the CD43++ GR-1(-) subset upon LPS stimulation, with 90% positivity and 5-fold higher fluorescence intensity at 1000 ng/ml LPS.
Conclusions:
- The murine CD43++ GR-1(-) monocyte subset exhibits pro-inflammatory characteristics.
- This subset is functionally homologous to human CD14+CD16+ monocytes.
- These findings provide insights into mouse monocyte subsets and their roles in immune responses.
