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Method for analysis dried vine fruits contaminated with ochratoxin A
Andrea C Galvis-Sánchez1, Antonio S Barros, Ivonne Delgadillo
1Departamento de Química, Universidade de Aveiro, Campus Universitário de Santiago, 3810-193 Aveiro, Portugal.
Analytica Chimica Acta
|May 20, 2008
Summary
Fourier infrared spectroscopy attenuated total reflection (FTIR-ATR) can detect ochratoxin A (OTA) in dried fruits. This method, using C18 cartridge pretreatment, shows good correlation for OTA detection within regulatory limits.
Area of Science:
- Food Science
- Analytical Chemistry
- Spectroscopy
Background:
- Ochratoxin A (OTA) is a harmful mycotoxin found in dried vine fruits.
- The EU has set a maximum limit of 10 µg/kg for OTA in dried vine fruits.
- Accurate and efficient detection methods for OTA are crucial for food safety.
Purpose of the Study:
- To evaluate Fourier infrared spectroscopy attenuated total reflection (FTIR-ATR) for OTA detection in dried vine fruits.
- To develop and validate a sample pretreatment method for OTA isolation.
- To assess the method's capability within the 2-50 µg/kg concentration range.
Main Methods:
- Sample pretreatment using a C18 cartridge for ochratoxin A isolation.
- Fourier infrared spectroscopy attenuated total reflection (FTIR-ATR) for spectral data acquisition.
- Partial Least Squares 1 (PLS1) analysis to correlate spectral data with OTA concentration.
Main Results:
- The C18 cartridge pretreatment effectively isolated ochratoxin A.
- FTIR-ATR combined with PLS1 analysis demonstrated a good correlation (R²=0.85) between spectral data and reference OTA concentrations.
- The method showed capability for detecting OTA in the 2-50 µg/kg range.
Conclusions:
- FTIR-ATR is a viable technique for detecting ochratoxin A in dried vine fruits.
- The developed method, including C18 pretreatment, offers a promising approach for routine food safety analysis.
- This spectroscopic method supports compliance with EU regulations for mycotoxin limits.
