Feasibility of using a surface plasmon resonance-based biosensor to detect and quantify yessotoxin

Area of Science:

• Marine Biology
• Analytical Chemistry
• Biochemistry

Background:

• Yessotoxin (YTX) is a marine toxin from dinoflagellates.
• YTX causes false positives in standard diarrhetic shellfish poisoning (DSP) toxin detection.
• Accurate YTX detection is crucial for food safety and regulatory limits.

Purpose of the Study:

• To develop a novel detection and quantification method for yessotoxin.
• To utilize Surface Plasmon Resonance (SPR) biosensor technology for YTX analysis.
• To establish an automated and reproducible assay for YTX detection.

Main Methods:

• Developed a Surface Plasmon Resonance (SPR)-based biosensor using immobilized phosphodiesterase enzymes (PDE I).
• Assayed yessotoxin (YTX) by measuring its interaction with PDE I.
• Established a dose-dependent calibration curve using YTX concentrations from 3-12 microM.

Main Results:

• Demonstrated a dose-dependent binding signal between YTX and immobilized PDE I.
• Achieved a linear calibration curve based on the observed rate constant (Kobs).
• The SPR biosensor method allows quantification of YTX at concentrations relevant to the European regulatory limit (1 mg kg(-1)).

Conclusions:

• The developed SPR biosensor provides an automated and repetitive method for YTX detection and quantification.
• This method offers improved accuracy for YTX analysis compared to traditional bioassays.
• The biosensor facilitates compliance with regulatory limits for YTX in seafood safety.