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Related Experiment Video

Updated: Jul 5, 2026

Antimicrobial Peptides Produced by Selective Pressure Incorporation of Non-canonical Amino Acids

Antimicrobial Peptides Produced by Selective Pressure Incorporation of Non-canonical Amino Acids

Published on: May 4, 2018

Synergistic effect between different milk-derived peptides and proteins.

I López-Expósito¹, A Pellegrini, L Amigo

¹Instituto de Fermentaciones Industriales, Consejo Superior de Investigaciones Científicas, Juan de la Cierva 3, 28006 Madrid, Spain.

Journal of Dairy Science

|May 20, 2008

Summary

Milk-derived antimicrobial peptides, bovine lactoferricin (LFcin-B) and bovine alpha(s2)-casein f(183-207), enhance the activity of nisin and bovine lactoferrin (LF). Synergistic effects were observed against foodborne pathogens like Listeria monocytogenes.

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Antimicrobial Peptides Produced by Selective Pressure Incorporation of Non-canonical Amino Acids

Antimicrobial Peptides Produced by Selective Pressure Incorporation of Non-canonical Amino Acids

Published on: May 4, 2018

Low Molecular Weight Protein Enrichment on Mesoporous Silica Thin Films for Biomarker Discovery

Low Molecular Weight Protein Enrichment on Mesoporous Silica Thin Films for Biomarker Discovery

Published on: April 17, 2012

Area of Science:

Food Science
Microbiology
Biochemistry

Background:

Antimicrobial peptides from food proteins are emerging as novel antimicrobial agents.
Bovine lactoferricin [lactoferrin f(17-41)] (LFcin-B) and bovine alpha(s2)-casein f(183-207) are key milk-derived peptides.
Nisin is a natural compound used in food preservation.

Purpose of the Study:

To investigate the enhancement of nisin's antimicrobial activity by milk-derived peptides.
To explore synergistic effects between milk-derived peptides and bovine lactoferrin (LF) against Escherichia coli and Staphylococcus epidermidis.
To evaluate the most effective combinations against foodborne pathogens Listeria monocytogenes and Salmonella choleraesuis.

Main Methods:

Assaying antimicrobial activity of combined agents.
Testing synergistic effects against specific bacterial strains (E. coli, Staph. epidermidis).
Evaluating efficacy against foodborne pathogens (L. monocytogenes, S. choleraesuis).

Main Results:

A synergistic antimicrobial effect was observed between LFcin-B and bovine LF against E. coli and Staph. epidermidis.
Bovine LF and nisin showed enhanced antimicrobial activity when combined with bovine alpha(s2)-casein f(183-207).
Synergistic effects were noted between LFcin-B and bovine LF, and between alpha(s2)-casein f(183-207) and nisin against L. monocytogenes.

Conclusions:

Combinations of milk-derived peptides with bovine LF or nisin can enhance antimicrobial efficacy.
LFcin-B and bovine LF exhibit synergistic activity, potentially mimicking in-vivo conditions.
The combination of alpha(s2)-casein f(183-207) and nisin offers a promising strategy against nisin-resistant L. monocytogenes.