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Related Experiment Videos

Compound Management for Quantitative High-Throughput Screening.

Adam Yasgar1, Paul Shinn, Ajit Jadhav

  • 1NIH Chemical Genomics Center, National Human Genome Research Institute, National Institutes of Health, Bethesda, MD 20892-3370, USA.

JALA (Charlottesville, Va.)
|May 23, 2008
PubMed
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The NIH Chemical Genomics Center developed a robust compound management system for high-throughput screening (HTS). This system efficiently handles over 200,000 compounds for quantitative HTS (qHTS) and lead development.

Area of Science:

  • Drug discovery and development
  • Chemical biology
  • High-throughput screening technologies

Background:

  • Efficient compound management is critical for high-throughput screening (HTS) and small molecule drug development.
  • The NIH Chemical Genomics Center (NCGC) requires a flexible and reliable system for handling a large compound library (>200,000 members).
  • The center employs quantitative HTS (qHTS), which necessitates preparing and tracking serial dilutions for concentration-response profiling.

Purpose of the Study:

  • To describe the compound management system and processes at the NCGC for HTS and probe development.
  • To detail the automation, equipment, and protocols for preparing inter-plate dilution series for qHTS.
  • To address specific challenges and solutions related to qHTS compound handling.

Main Methods:

Related Experiment Videos

  • Selection and implementation of automated equipment for parallel compound manipulation in 384- and 1,536-well formats.
  • Development of protocols for preparing inter-plate dilution series (vertical titrations).
  • Registration and tracking systems for managing compound plates and dilutions.

Main Results:

  • A reliable and automated system for compound manipulation in multiple well formats was established.
  • Protocols for efficient preparation of serial dilutions for qHTS assays were implemented.
  • The system enables effective tracking and management of a large compound library for screening purposes.

Conclusions:

  • The described compound management system supports the NCGC's qHTS and probe development initiatives.
  • Automation and standardized protocols enhance the efficiency and reliability of compound handling.
  • This system is crucial for the success of downstream processes in drug discovery.