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Automated analytical microarrays: a critical review.
Michael Seidel1, Reinhard Niessner
1Chair for Analytical Chemistry and Institute of Hydrochemistry, Technische Universität München, Marchioninistrasse 17, 81377, München, Germany. michael.seidel@ch.tum.de
Analytical and Bioanalytical Chemistry
|May 28, 2008
Summary
Microarrays enable simultaneous detection of multiple analytes using antibody-based immunoassays. This review highlights automated systems and quantitative multiplexed applications for diverse analytical needs.
Area of Science:
- Biotechnology
- Analytical Chemistry
- Immunology
Background:
- Microarrays offer simultaneous detection of multiple analytes via specific affinity reactions.
- Antibody-based immunoassays are versatile for detecting small molecules, biopolymers, and complex biological structures.
Purpose of the Study:
- To review current research on microarrays, focusing on automated systems.
- To explore quantitative multiplexed applications of microarrays in analytical fields.
Main Methods:
- Utilizing antibody-antigen interactions for multiplexed quantitative analysis.
- Developing diverse readout systems including fluorescence, chemiluminescence, and electrochemical methods.
- Integrating microarrays with fluidic systems for flow-through applications.
Main Results:
- Antibody-based microarrays can detect a wide range of analytes, from small molecules to pathogens.
- Various advanced readout technologies have been developed for microarray analysis.
- Automated and validated systems are crucial for widespread acceptance of microarrays.
Conclusions:
- Microarrays, particularly immunoanalytical ones, show significant potential for food/water analysis and clinical diagnostics.
- The development of robust, automated platforms is key to realizing the full potential of microarray technology.
- Future research focuses on enhancing automation and validation for reliable quantitative multiplexed analysis.
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