Related Experiment Videos
Extracellular ATP itself elicits superoxide generation in guinea pig peritoneal macrophages
M Nakanishi1, H Takihara, Y Minoru
1Second Department of Internal Medicine, Faculty of Medicine, Fukuoka University, Japan.
FEBS Letters
|April 22, 1991
Abstract:
Extracellular ATP itself elicited the generation of superoxide (O2-) in guinea pig peritoneal macrophages associated with an increase in cytosolic calcium ([Ca2+]i). The ATP-induced O2- generation was completely inhibited by pretreatment with pertussis toxin (PT) accompanied by the suppression of [Ca2+]i mobilization. Pre-exposure to a small amount of phorbol myristate acetate (PMA) primed the ATP-induced generation of O2- without a change of [Ca2+]i. The results suggest that ATP-induced O2- generation is mediated by [Ca2+]i mobilization and by PT-sensitive G protein.