[Determination of five primary chemical constituents in Cassia angustifolia by HPLC]
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Updated: Jul 4, 2026
A Generalized Method for Determining Free Soluble Phenolic Acid Composition and Antioxidant Capacity of Cereals and Legumes
Published on: June 10, 2022
Qiu-Ping Wu1, Zhu-Ju Wang, Li-Ying Tang
1Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
Objective:
To establish a method for determining the content of primary chemical constituents in the leaves of Cassia angustifolia.
Method:
The HPLC with Diamonsil C18 (4.6 mm x 250 mm, 5 microm) column was used , acetonitrile-1% acetic acid (10:90-15: 85-18: 82-20: 80-25: 75) in a gradient manner was used as a mobile phase, with flow rate of 1 mL x min(-1), column temperature at 40 degrees C and detection wavelength at 270 nm.
Result:
The results showed that 5 effective components all separated well and showed good linearity.
Conclusion:
The method was proved to be rapid, sensitive, accurate, credible and repeatable. It can be applied to quality control of Folium Sennae.
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