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Related Experiment Video

Updated: Jul 4, 2026

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Published on: December 9, 2013

Subdiffraction multicolor imaging of the nuclear periphery with 3D structured illumination microscopy.

Lothar Schermelleh¹, Peter M Carlton, Sebastian Haase

¹Center for Integrated Protein Science, Department of Biology, Ludwig Maximilians University Munich, 82152 Planegg-Martinsried, Germany.

Science (New York, N.Y.)

|June 7, 2008

Summary

Three-dimensional structured illumination microscopy (3D-SIM) overcomes resolution limits for detailed cellular imaging. This advanced technique reveals previously unseen nuclear structures, including nuclear pore complexes and nuclear envelope features.

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Super-resolution Imaging of the Cytokinetic Z Ring in Live Bacteria Using Fast 3D-Structured Illumination Microscopy (f3D-SIM)

Super-resolution Imaging of the Cytokinetic Z Ring in Live Bacteria Using Fast 3D-Structured Illumination Microscopy (f3D-SIM)

Published on: September 29, 2014

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Last Updated: Jul 4, 2026

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Published on: December 9, 2013

Super-resolution Imaging of the Cytokinetic Z Ring in Live Bacteria Using Fast 3D-Structured Illumination Microscopy (f3D-SIM)

Super-resolution Imaging of the Cytokinetic Z Ring in Live Bacteria Using Fast 3D-Structured Illumination Microscopy (f3D-SIM)

Published on: September 29, 2014

Area of Science:

Cell Biology
Microscopy Techniques
Molecular Imaging

Background:

Conventional fluorescence microscopy is limited by spatial resolution, hindering detailed visualization of subcellular structures.
Understanding the mammalian nucleus requires advanced imaging to resolve complex molecular organization.

Purpose of the Study:

To overcome the diffraction limit of light microscopy for enhanced visualization of the mammalian nucleus.
To simultaneously image chromatin, nuclear lamina, and nuclear pore complexes (NPCs) with high resolution.

Main Methods:

Application of three-dimensional structured illumination microscopy (3D-SIM) for multicolor imaging.
Simultaneous visualization of chromatin, nuclear lamina, and NPCs.

Main Results:

Resolved single NPCs colocalizing with lamin network channels and peripheral heterochromatin.
Differentiated localization of distinct NPC components.
Detected double-layered nuclear envelope invaginations in prophase, previously observed only by electron microscopy.

Conclusions:

3D-SIM significantly enhances the study of subcellular structures beyond the diffraction limit.
This technique provides new facile possibilities for analyzing nuclear organization and dynamics.
Multicolor 3D-SIM offers unprecedented insights into the mammalian nucleus.