Liposomes formed in sintered glass pores

The method for preparation of vesicles, by evaporation of hydrophobic solvent from double emulsion (w/o/w) formed in the properly designed device is described. These method leads to multiple increase of encapsulation efficiency of aqueous solutions of drug in liposomes in comparison with other method. The w/o/w was passed through the glass sinter with the use of negative pressure to disrupt w/o/w drops into smaller ones. At low pressure and at heigher temperature, the hydrophobic solvent from oil phase evaporated off and the lipids that were diluted in oil phase had created bilayer. When the relatively small quantity of lipids was used, the final encapsulation efficiency (ee) was about 50% and the uppermost encapsulation volume (ev) was 160 mL/g of lipids. Similar ee was noted for a 4-amino-10-methylfolic acid (MTX), Patent Blue V (PB) and bovine serum albumin (BSA). Liposomes loaded with drug at high concentration may be easily separated from suspension with the use of simple centrifugation.