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Antiviral Nucleoside Inhibitors01:22

Antiviral Nucleoside Inhibitors

Antiviral Nucleoside InhibitorsAntiviral nucleoside inhibitors are structural analogs of natural nucleosides that interfere with viral DNA or RNA synthesis. These compounds selectively target viral polymerases due to their resemblance to host nucleosides, thereby disrupting viral genome replication.Mechanism of Acyclovir ActionAcyclovir is a guanosine analog with a three-carbon acyclic side chain. It selectively targets herpes simplex virus type 1 (HSV-1), herpes simplex virus type 2 (HSV-2),...
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Nucleic acid biosynthesis is a fundamental biochemical process that produces the purine and pyrimidine nucleotides essential for DNA and RNA synthesis. This pathway maintains a balanced nucleotide pool, preventing imbalances that could jeopardize genetic integrity and cellular function. Given the crucial role of nucleotides, their synthesis is tightly regulated to ensure proper cellular homeostasis.Purine BiosynthesisThe biosynthesis of purine nucleotides begins with ribose-5-phosphate, a...
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Phosphodiester bond forms when a phosphoric acid molecule (H3PO4) links with two hydroxyl groups (–OH) of two other molecules, forming two ester bonds. Two water molecules are released in this process. The phosphodiester bond is commonly found in nucleic acids (DNA and RNA) and plays a critical role in their structure and function.
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Nucleoside Triphosphates - From Synthesis to Biochemical Characterization
15:22

Nucleoside Triphosphates - From Synthesis to Biochemical Characterization

Published on: April 3, 2014

Solid-supported diphosphitylating and triphosphitylating reagents for nucleoside modification.

Yousef Ahmadibeni1, Keykavous Parang

  • 1University of Rhode Island, Kingston, Rhode Island, USA.

Current Protocols in Nucleic Acid Chemistry
|June 14, 2008
PubMed
Summary

This study introduces novel polymer-bound reagents for synthesizing nucleoside diphosphates and triphosphate analogs. This solid-phase method simplifies the preparation of phosphorylated compounds and oligonucleotides.

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Protocol for the Solid-phase Synthesis of Oligomers of RNA Containing a 2'-O-thiophenylmethyl Modification and Characterization via Circular Dichroism
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Protocol for the Solid-phase Synthesis of Oligomers of RNA Containing a 2'-O-thiophenylmethyl Modification and Characterization via Circular Dichroism

Published on: July 28, 2017

Area of Science:

  • Organic Chemistry
  • Biochemistry
  • Synthetic Chemistry

Background:

  • Nucleoside phosphates are crucial for biological processes and therapeutics.
  • Existing synthesis methods often require protected nucleosides and intermediate purification.

Purpose of the Study:

  • To develop a streamlined solid-phase synthesis for nucleoside 5'-O-diphosphate and 5'-O-triphosphate analogs.
  • To enable the preparation of oligonucleotides with diphosphodiester internucleotide bridges.

Main Methods:

  • Immobilization of diphosphitylating and triphosphitylating reagents onto polymer-bound linkers.
  • Selective reaction with the 5'-hydroxyl group of nucleosides.
  • Oxidation, deprotection, and cleavage from the resin to yield final products.

Main Results:

  • Successful synthesis of various nucleoside 5'-O-diphosphate and 5'-O-triphosphate analogs.
  • Demonstrated utility in preparing oligodeoxynucleotides with diphosphodiester linkages.
  • Eliminated the need for nucleoside phosphate precursors and intermediate purification.

Conclusions:

  • The described solid-phase strategy offers an efficient and simplified approach for synthesizing phosphorylated nucleoside analogs.
  • This method facilitates the construction of modified oligonucleotides without complex precursor requirements.