Related Experiment Video
Updated: Oct 23, 2026

Analytical Techniques for Assaying Nitric Oxide Bioactivity
Published on: June 18, 2012
Activation of N'-nitrosonornicotine by hydrogen peroxide in vitro
J Nair1, U J Nair, A J Amonkar
1Carcinogenesis Division, Tata Memorial Centre, Parel, Bombay, India.
Abstract:
Betel-quid ingredients were found to produce reactive oxygen species, such as superoxide anion and hydrogen peroxide, in vitro. We demonstrated that N'-nitrosonornicotine (NNN) can be converted to its active metabolite, hydrogen peroxide, nonenzymatically in the presence of ferrous ions and ethylenediaminetetracetic acid (EDTA) at pH 7.2. Three ultimate metabolites of NNN--NNN-1-N-oxide, 4-hydroxy-4-(3- pyridyl)butyric acid and 4-oxo-4-(3-pyridyl)butyric acid--and nornicotine were detected by high-performance liquid chromatography. 3H-NNN and 14C-NNN interact with calf thymus DNA in the presence of hydrogen peroxide, ferrous ion and EDTA. The results suggest that formation of reactive oxygen species in the presence of NNN may be a key factor in the initiation of oral tumours in tobacco and betel-quid chewers.
More Related Videos
07:38A General Method for Detecting Nitrosamide Formation in the In Vitro Metabolism of Nitrosamines by Cytochrome P450s
Published on: September 25, 2017
08:23Chemiluminescence-based Assays for Detection of Nitric Oxide and its Derivatives from Autoxidation and Nitrosated Compounds
Published on: February 16, 2022
Related Concept Videos
meta-Directing Deactivators: –NO2, –CN, –CHO, –⁠CO2R, –COR, –CO2H
Preparation of Amines: Reduction of Oximes and Nitro Compounds
Though catalytic hydrogenation can reduce nitrobenzenes, the reduction is nonselective in the presence of other functional groups. For instance, if nitrobenzene contains an aldehyde group,...
1° Amines to Diazonium or Aryldiazonium Salts: Diazotization with NaNO2 Overview
The nitrous acid is unstable. Hence, it is formed in situ from a solution of sodium nitrite and cold aqueous acids such as hydrochloric or sulfuric acid. In an acidic solution, the –OH group of nitrous acid undergoes protonation to give oxonium ion, followed by water loss...
2° Amines to N-Nitrosamines: Reaction with NaNO2
Nitrosation of Enols
Bioactivation and Tissue Toxicity