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Updated: Jul 4, 2026

DNA-affinity-purified Chip (DAP-chip) Method to Determine Gene Targets for Bacterial Two component Regulatory Systems
Published on: July 21, 2014
Relative expression of genes involved in the resistance/sensitivity of Enterococcus faecalis JH2-2 to recombinant
Ségolène Calvez1, H Prevost, Djamel Drider
1Laboratoire de Microbiologie, ENITIAA, Rue de la Géraudière, BP 82225, 44322 Nantes Cedex 3, France.
Abstract:
The relative expression of mptABCD operon, glpQ, pde, rpoN, mptR and gap-1 was studied by reverse transcription combined with the real time polymerase chain reaction to understand the role of each gene in resistance/sensitivity of Enterococcus faecalis to class IIa bacteriocins such as recombinant divercin V41 (DvnRV41). Comparative critical threshold methods in presence or absence of DvnRV41 were then used to determine the level of expression of each gene cited above. In the presence of DvnRV41, the rpoN and glpQ genes were down-regulated, mptR, mptC, gap-1 and pde genes were up-regulated, whilst expression of mptB and mptD genes remained unmodified.
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