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Ultrafast Lignin Extraction from Unusual Mediterranean Lignocellulosic Residues
Published on: March 9, 2021
One-dimensional and two-dimensional liquid chromatography of sulphonated lignins
Stella Brudin1, James Berwick, Mike Duffin
1Polymer-Analysis Group (HIMS), University of Amsterdam, Nieuwe Achtergracht 166, 1018 WV Amsterdam, The Netherlands. S.S.Brudin@uva.nl
Journal of Chromatography. A
|June 25, 2008
Summary
Analyzing lignosulphonates and sulphonated kraft lignins is crucial for agrochemical formulations. This study developed advanced chromatography methods to link lignin properties like size and sulfonation to formulation stability.
Area of Science:
- Analytical Chemistry
- Polymer Chemistry
- Agrochemical Science
Background:
- Sulphonated lignins are vital dispersants in agrochemical formulations.
- Certain lignosulphonates can cause physical instabilities in these formulations.
- Understanding lignin properties is key to optimizing formulation performance.
Purpose of the Study:
- To develop and apply advanced chromatographic techniques for analyzing lignosulphonates and sulphonated kraft lignins.
- To identify specific structural properties of sulphonated lignins that influence agrochemical formulation characteristics.
- To establish correlations between lignin structure and performance as dispersants.
Main Methods:
- Development of one- and two-dimensional separation methods.
- Application of ion-pair reversed-phase liquid chromatography (IP-RPLC) with tetrapentylammonium bromide.
- Utilisation of aqueous size-exclusion chromatography (SEC).
- Combination of RPLC and SEC into a comprehensive two-dimensional liquid chromatography (LCxLC) system.
Main Results:
- Observed significant differences in size distribution among various sulphonated lignin batches.
- Found high correlation between retention times in the two chromatographic dimensions, limiting the full potential of LCxLC.
- Demonstrated that IP-RPLC retention is primarily determined by molecular size, not the degree of sulphonation for similar-sized molecules.
- Indicated that the degree of sulphonation is relatively constant across separated molecules of similar size.
Conclusions:
- The study provides critical insights into the structural characteristics of sulphonated lignins relevant to agrochemical formulations.
- Developed chromatographic methods offer a pathway to better understand and predict formulation stability based on lignin properties.
- This research is a significant step towards correlating lignin oligomer structure (size, sulfonation) with surfactant requirements in agrochemical applications.
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