Related Experiment Video
Updated: Jul 4, 2026
06:53
Preparation of Food Samples Using Homogenization and Microwave-Assisted Wet Acid Digestion for Multi-Element Determination with ICP-MS
Published on: December 22, 2023
Ultrasonic-assisted enzymatic digestion (USAED) for total elemental determination and elemental speciation: a
1REQUIMTE, Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, 2829-516 Monte da Caparica, Portugal.
Talanta
|July 1, 2008
Summary
Ultrasonic-Assisted Enzymatic Digestion (USAED) offers a novel sample preparation method for elemental analysis. This review covers recent advancements, critical parameters, and practical guidance for its application in analytical chemistry.
Area of Science:
- Analytical Chemistry
- Materials Science
Background:
- Traditional sample preparation methods can be time-consuming and may introduce errors.
- Enzymatic digestion is a valuable technique for breaking down complex matrices.
- Ultrasonic technology can enhance extraction efficiency and reduce processing times.
Purpose of the Study:
- To review recent achievements in Ultrasonic-Assisted Enzymatic Digestion (USAED) for elemental determination and speciation.
- To identify and discuss critical variables affecting USAED performance.
- To provide a practical guide and insights into future trends for USAED.
Main Methods:
- Literature review of recent publications on USAED.
- Analysis of factors influencing enzymatic activity and ultrasonic efficiency.
- Compilation of practical considerations and error management strategies.
Main Results:
- USAED significantly accelerates solid-liquid extraction for elemental analysis.
- Enzyme type and ultrasonic system parameters are key factors for optimization.
- Common data interpretation errors and their mitigation are discussed.
- A guide for rapid USAED application is presented.
Conclusions:
- USAED is a promising and efficient sample treatment technique in analytical chemistry.
- Understanding critical parameters and potential errors is crucial for reliable results.
- Future research should focus on further optimizing USAED protocols and expanding its applications.
Related Concept Videos
01:21Atomic Absorption Spectroscopy: Lab
For AAS measurements, samples must be introduced as clear solutions, often requiring extensive preliminary treatment to dissolve materials like soils, animal tissues, and minerals. Common methods for sample preparation include treatment with hot mineral acids, wet ashing, combustion in closed containers, high-temperature ashing, or fusion with reagents.
Solutions containing organic solvents, such as low-molecular-mass alcohols, esters, or ketones, enhance absorbances by increasing nebulizer...
Solutions containing organic solvents, such as low-molecular-mass alcohols, esters, or ketones, enhance absorbances by increasing nebulizer...
01:20Atomic Emission Spectroscopy: Overview
Atomic emission spectroscopy (AES) is an analytical technique used to determine the elemental composition of a sample by analyzing the light emitted from excited atoms. In AES, atoms in a sample are excited to higher energy levels by thermal energy from high-temperature sources, such as plasma, arcs, or sparks. When these excited atoms return to lower energy states, they emit light at specific wavelengths characteristic of each element. The resulting atomic emission spectrum, which consists of...
01:08Sample Preparation for Analysis: Advanced Techniques
Accurate analysis of complex samples often requires advanced preparation techniques to achieve reliable and reproducible results. Samples containing inorganic or organic materials can be challenging to dissolve or decompose effectively. Standard sample preparation methods include acid digestion, fusion, dry ashing, and wet digestion.
Acid digestion with strong acids is commonly used to dissolve inorganic materials that are insoluble (do not dissolve) in water. This method can be useful for...
Acid digestion with strong acids is commonly used to dissolve inorganic materials that are insoluble (do not dissolve) in water. This method can be useful for...
01:27Atomic Absorption Spectroscopy: Overview
Atomic absorption spectroscopy (AAS) is a technique used to analyze elements by measuring electromagnetic radiation (EMR) absorbed by atoms, which causes them to transition to a higher-energy orbit. The most crucial step in AAS is atomization, where the analyte is converted into gas-phase atoms, typically through a flame or furnace. Some of these atoms become thermally excited in the flame, while most remain in the ground state.
When irradiated by EMR of a particular wavelength, these...
When irradiated by EMR of a particular wavelength, these...
01:21Tandem Mass Spectrometry
Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and reduce chemical noise during analyte detection. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called...
01:22Atomic Emission Spectroscopy: Instrumentation
The instrumentation of atomic emission spectrometry (AES) involves various components, including atomization devices that convert samples into gas-phase atoms and ions. There are two main types of atomization devices: continuous and discrete atomizers. Continuous atomizers, like plasmas and flames, introduce samples in a constant stream, while discrete atomizers inject individual samples using syringes or autosamplers. The most common discrete atomizer is the electrothermal atomizer.