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Updated: Jul 4, 2026

Production and Targeting of Monovalent Quantum Dots
Published on: October 23, 2014
NHS-mediated QDs-peptide/protein conjugation and its application for cell labeling
Yaming Shan1, Liping Wang, Yuhua Shi
1College of Life Science, Jilin University, Jiefang Road 115, Changchun 130023, PR China.
Abstract:
3-Mercaptopropyl acid-stabilized CdTe nanoparticles synthesized in aqueous solution are found to be able to conjugate with peptides or proteins mediated by N-hydroxysulfo-succinimide (NHS) but 1-ethyl-3(3-dimethylaminopropyl) carbodiimides hydrochloride (EDC). The reaction time and pH have been optimized. Gel-permeation HPLC was applied following the conjugation, which could quickly and simultaneously detect and purify the quantum dots (QDs) conjugates. The biological activities of QDs conjugates are maintained and give superior results in cell labeling. These results are encouraging regarding the application of QDs molecules for use in living cells, diagnostics and drug delivery.
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