Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Modern Molecular Taxonomy

Modern Molecular Taxonomy

Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...

DNA Microarrays

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

Applications of Molecular Taxonomy

Applications of Molecular Taxonomy

Molecular taxonomy has revolutionized the understanding and classification of bacteria, providing precise insights into their diversity, evolutionary relationships, and ecological roles. By utilizing molecular techniques such as DNA sequencing and fingerprinting, researchers have made significant strides in various fields related to bacterial studies.Resolving Taxonomic AmbiguitiesMolecular taxonomy has been instrumental in distinguishing closely related bacterial species initially thought to...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Intraspecific diversity of <i>Staphylococcus aureus</i> populations isolated from cystic fibrosis respiratory infections.

Microbiology spectrum·2026

Same author

Host and antibiotic jointly select for greater virulence in <i>Staphylococcus aureus</i>.

eLife·2026

Same author

Phylogenetic diversity, functional pathways, and network interactions of ocular chlamydia-like organisms (CLOs) in trachoma-endemic Ethiopia.

mBio·2026

Same author

camlhmp: a simple framework for building reproducible microbial genome-based typing tools.

Microbiology resource announcements·2026

Same author

Characterizing Oral Microbiome and Periodontal Disease in Oral HPV-Positive (COMP-HPV) individuals with HIV: an observational longitudinal study protocol.

BMC oral health·2026

Same author

CtGAP 2.0: enhanced genome assembly, strain resolution, and phylogenomics for <i>Chlamydia trachomatis</i>.

Microbiology resource announcements·2026

Search research articles

Related Experiment Video

Updated: Jul 4, 2026

Demonstrating a Multi-drug Resistant Mycobacterium tuberculosis Amplification Microarray

Demonstrating a Multi-drug Resistant Mycobacterium tuberculosis Amplification Microarray

Published on: April 25, 2014

Genotyping of Bacillus cereus strains by microarray-based resequencing.

Michael E Zwick¹, Maureen P Kiley, Andrew C Stewart

¹Biological Defense Research Directorate, Naval Medical Research Center, Silver Spring, Maryland, United States of America. Michael E. Zwick

|July 4, 2008

Summary

Custom resequencing arrays (RAs) efficiently distinguish Bacillus anthracis from related B. cereus strains. This genotyping technology aids in pre-screening large collections for whole genome sequencing.

More Related Videos

Competitive Genomic Screens of Barcoded Yeast Libraries

Competitive Genomic Screens of Barcoded Yeast Libraries

Published on: August 11, 2011

Characterization of a Pathogenic Escherichia coli Strain Derived from Oreochromis spp. Farms Using Whole-Genome Sequencing

Characterization of a Pathogenic Escherichia coli Strain Derived from Oreochromis spp. Farms Using Whole-Genome Sequencing

Published on: December 23, 2022

Related Experiment Videos

Last Updated: Jul 4, 2026

Demonstrating a Multi-drug Resistant Mycobacterium tuberculosis Amplification Microarray

Demonstrating a Multi-drug Resistant Mycobacterium tuberculosis Amplification Microarray

Published on: April 25, 2014

Competitive Genomic Screens of Barcoded Yeast Libraries

Competitive Genomic Screens of Barcoded Yeast Libraries

Published on: August 11, 2011

Characterization of a Pathogenic Escherichia coli Strain Derived from Oreochromis spp. Farms Using Whole-Genome Sequencing

Characterization of a Pathogenic Escherichia coli Strain Derived from Oreochromis spp. Farms Using Whole-Genome Sequencing

Published on: December 23, 2022

Area of Science:

Microbiology
Genomics
Bioinformatics

Background:

Distinguishing microbial pathogens from nonpathogenic relatives is crucial for understanding population biology.
Bacillus anthracis, the cause of inhalational anthrax, is closely related to the B. cereus group, making differentiation challenging.

Purpose of the Study:

To develop and evaluate a genotyping technology for discriminating between Bacillus anthracis and other B. cereus sensu lato strains.
To assess the utility of resequencing arrays (RAs) for analyzing genetic diversity in bacterial collections.

Main Methods:

Custom-designed resequencing arrays (RAs) were developed based on the Bacillus anthracis genome sequence.
Genomic sequence (422 kb) was generated from a panel of 41 B. cereus sensu lato strains using RAs.

Main Results:

RAs demonstrated a "one reaction" capability for genotyping.
The technology effectively discriminated between highly similar B. anthracis isolates and more divergent B. cereus s.l. Clade 1 strains.

Conclusions:

Resequencing arrays are an efficient genotyping technology for bacterial strain collections.
RAs can be used to pre-screen genetic diversity and select optimal candidates for whole genome sequencing.