Related Experiment Video

Updated: Jul 4, 2026

High-throughput Assay to Phenotype Salmonella enterica Typhimurium Association, Invasion, and Replication in Macrophages
11:10

High-throughput Assay to Phenotype Salmonella enterica Typhimurium Association, Invasion, and Replication in Macrophages

Published on: August 11, 2014

[Interaction of a causative agent with associative bacteria during salmonellosis]

O V Bukharin, A V Valyshev, E V Ivanova

    Zhurnal Mikrobiologii, Epidemiologii I Immunobiologii
    |July 4, 2008
    PubMed
    Abstract

    Insights

    Salmonellosis alters gut microflora, reducing beneficial bifidobacteria and increasing yeast. Beneficial bacteria decrease Salmonella

    Area of Science:

    • Microbiology
    • Gut Microbiome Research
    • Infectious Diseases

    Background:

    • Salmonellosis significantly impacts gut microbial composition.
    • The persistent characteristics of microorganisms, such as antilysozyme activity (ALA), are crucial in symbiotic associations.
    • Understanding these interactions is key to managing dysbiosis.

    Purpose of the Study:

    • To characterize gut microflora during salmonellosis.
    • To investigate how symbiotic microorganisms' antilysozyme activity (ALA) is modified in associations.
    • To explore the role of gut microbiota in Salmonella persistence.

    Main Methods:

    • Bacteriological fecal analysis of 90 individuals (salmonellosis patients and healthy controls).
    • Isolation of microorganisms associated with Salmonella infection.
    • Assessment of the influence of isolated microbes on Salmonella's ALA.

    Main Results:

    • Gut microbiocenosis was more diverse in patients than in healthy individuals.
    • A significant reduction in bifidobacteria (to ≤10^7 CFU/g) was observed, particularly during the reconvalescent phase.
    • Bifidoflora deficiency correlated with an increase in yeast fungi. Exometabolites from bifidobacteria decreased Salmonella ALA, while enterobacteria and staphylococci stimulated it.

    Conclusions:

    • Gut microbiota composition is significantly altered during salmonellosis.
    • Bacterial interactions within the gut influence Salmonella's persistence and antilysozyme activity.
    • These findings provide insights into pathobiocenosis formation and bacterial carriage mechanisms.

    More Related Videos

    Automated Analysis of Intracellular Phenotypes of Salmonella Using ImageJ10:39

    Automated Analysis of Intracellular Phenotypes of Salmonella Using ImageJ

    Published on: August 9, 2022

    Chronic Salmonella Infection Induced Intestinal Fibrosis08:40

    Chronic Salmonella Infection Induced Intestinal Fibrosis

    Published on: September 22, 2019

    Related Concept Videos

    Bacterial Gastroenteritis01:18

    Bacterial Gastroenteritis

    Bacterial gastroenteritis, characterized by diarrhea, abdominal cramps, and vomiting, is often caused by ingestion of contaminated food or water and is frequently associated with pathogenic Escherichia coli strains. These microbes exploit two principal mechanisms to inflict disease.Shiga toxin–producing E. coli, also referred to as STEC—notably O157:H7—release Shiga toxins that target ribosomes, blocking protein synthesis. The B subunit of the toxin binds the host glycolipid receptor...
    Reservoir of Infection01:30

    Reservoir of Infection

    Infectious diseases arise from intricate interactions between pathogens and their reservoirs. A reservoir of infection refers to the natural habitat where a pathogen lives, grows, and multiplies, serving as a continual source of infection. Reservoirs are broadly classified as either living or nonliving, and each plays a unique role in disease transmission, significantly influencing public health interventions and control strategies.Humans act as reservoirs for a wide array of pathogens,...
    Determinants of Bacterial Pathogenicity and Virulence01:20

    Determinants of Bacterial Pathogenicity and Virulence

    Pathogenic bacteria employ a variety of strategies to establish infections, including the secretion of extracellular enzymes that act as potent virulence factors. These enzymes facilitate bacterial colonization of host tissues and help evade immune surveillance. By targeting structural components of host tissues and interfering with immune mechanisms, these enzymes play a pivotal role in disease progression.Extracellular Enzymes Facilitating Tissue Invasion: Several bacterial pathogens secrete...
    Cholera01:25

    Cholera

    Cholera is an acute gastrointestinal disease caused by the Gram-negative bacterium Vibrio cholerae. It is transmitted primarily via the fecal-oral route through the ingestion of contaminated water or food.Vibrio cholerae is a motile, Gram-negative bacterium of the family Vibrionaceae, primarily associated with waterborne outbreaks in areas with inadequate sanitation. Although over 200 serogroups of V. cholerae exist, only O1 and O139 are responsible for epidemic cholera. The O1 serogroup,...
    Colonisation of Pathogens01:25

    Colonisation of Pathogens

    Pathogen colonization of host tissues is a critical step in the development of infectious diseases. Various pathogenic microorganisms, including bacteria, fungi, viruses, and protozoa, have evolved complex strategies to attach to, invade, and persist within host environments. These mechanisms enable pathogens to establish infections, evade immune responses, and resist antimicrobial treatments.Attachment to Host CellsIn bacteria, colonization typically begins with adherence to host epithelial...
    Lysogenic Cycle of Bacteriophages00:43

    Lysogenic Cycle of Bacteriophages

    In contrast to the lytic cycle, phages infecting bacteria via the lysogenic cycle do not immediately kill their host cell. Instead, they combine their genome with the host genome, allowing the bacteria to replicate the phage DNA along with the bacterial genome. The incorporated copy of the phage genome is called the prophage. Some prophages can re-activate and enter the lytic cycle. This often occurs in response to a perturbation, such as DNA damage, but can also transpire in the absence of...