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Updated: Jul 3, 2026

A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions

A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions

Published on: November 23, 2015

Imaging of the cell surface interface using objective coupled widefield surface plasmon microscopy.

M Mahadi Abdul Jamil¹, M C T Denyer, M Youseffi

¹Bradford University, School of Life Sciences, School of Engineering, Design & Technology and Institute of Pharmaceutical Innovation, Bradford, West Yorkshire, UK.

Journal of Structural Biology

|July 10, 2008

Summary

The new widefield surface plasmon resonance (WSPR) microscope images cell surfaces at high resolution without labels. This advanced microscopy technique allows for the study of live cell interactions and signaling mechanisms at the cell surface interface.

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Area of Science:

Biophysics
Cell Biology
Microscopy

Background:

Surface plasmon resonance (SPR) microscopy offers nanometric resolution for studying interfaces.
Existing SPR systems provide high resolution but can be limited in live-cell applications.

Purpose of the Study:

To develop and demonstrate the utility of a novel widefield surface plasmon resonance (WSPR) microscope.
To investigate the cell surface interface with submicron lateral resolution using WSPR microscopy.
To assess WSPR's capability for imaging both fixed and live cells without labeling.

Main Methods:

Development of a Kohler-illuminated WSPR microscope utilizing high numerical aperture (NA) oil immersion objectives (1.45 NA and 1.65 NA).
Excitation of surface plasmons at the gold-dielectric interface using different objective lenses.
Imaging of fixed HaCaT cells in air and live HaCaT cells in culture media.

Main Results:

High-resolution, high-contrast imaging of submicron features, including vinculin in focal adhesions, was achieved with fixed cells.
Time-lapse video WSPR microscopy enabled tracking of cell surface interfacial interactions in live cells.
Demonstrated label-free interrogation of the cell surface interface and cellular signaling in live cells.

Conclusions:

The widefield surface plasmon resonance microscope provides a powerful tool for label-free investigation of cell surface dynamics.
WSPR microscopy facilitates the study of cellular interactions and signaling pathways in real-time within live cells.
This technology opens new avenues for understanding cell behavior at the interface without the need for fluorescent or other labeling techniques.