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Published on: July 18, 2017
Therapeutic effects of cefluprenam (CFLP) on polymicrobial infections associated with Enterococcus faecalis in rat
1Department of Obstetrics and Gynecology, School of Medicine, Gifu University, 40, Tsukasa-machi, Gifu-city, Gifu 500, Japan.
Abstract:
Enterococcus faecalis plays an important role as one of the pathogens in polymicrobial infections. We evaluated the efficacy of cefluprenam (CFLP) using a polymicrobial pyometra of a model rat. Rats were infected with a mixed intrauterine inoculation of E. faecalis plus either Bacteroides fragilis or Prevotella bivia (minimal inhibitory concentration of CFLP: E. faecalis, 3.13 mug/ml; B. fragilis, 3.13 mug/ml; P. bivia, 3.13 mug/ml). Immediately after inoculating 10(5) cfu/rat of each organism, CFLP (either 40 mg/kg, i.v., q.i.d. for 5 days or 80 mg/kg, i.v., b.i.d. for 5 days) was administered. The intrauterine inflammatory change and bacterial count in the treated group were compared with those in the non-treated control group. CFLP significantly (P < 0.01) decreased the bacterial counts except for B. fragilis in the regimen of 80 mg/kg, b.i.d. However, the regimen of 40 mg/kg, q.i.d. significantly (P < 0.05) reduced the bacterial counts more than did that of 80 mg/kg, b.i.d. CFLP proved to demonstrate a good tissue concentration above 3 mug/g for 1 h. These results suggest that CFLP in a more divided dose is efficacious for the treatment of polymicrobial infections associated with E. faecalis in pyometra.
Insights
Cefluprenam (CFLP) effectively treated polymicrobial pyometra in rats, particularly when administered in a more divided dosage. This antibiotic demonstrated significant bacterial count reduction, suggesting efficacy in complex infections.
Area of Science:
- Microbiology
- Pharmacology
- Infectious Diseases
Background:
- Enterococcus faecalis is a key pathogen in polymicrobial infections.
- Polymicrobial pyometra presents a complex therapeutic challenge.
- Evaluating novel antibiotic efficacy in polymicrobial models is crucial.
Purpose of the Study:
- To assess the efficacy of cefluprenam (CFLP) against polymicrobial pyometra in a rat model.
- To compare different dosing regimens of CFLP for treating intrauterine infections.
Main Methods:
- A rat model of polymicrobial pyometra was established using Enterococcus faecalis combined with either Bacteroides fragilis or Prevotella bivia.
- Rats were treated with cefluprenam (CFLP) at 40 mg/kg q.i.d. or 80 mg/kg b.i.d. intravenously for 5 days.
- Bacterial counts and intrauterine inflammatory changes were assessed and compared to a control group.
Main Results:
- CFLP significantly reduced bacterial counts in most cases, except for B. fragilis with the 80 mg/kg b.i.d. regimen.
- The 40 mg/kg q.i.d. regimen showed significantly greater bacterial reduction compared to the 80 mg/kg b.i.d. regimen.
- CFLP achieved substantial tissue concentrations (>3 mug/g) for at least 1 hour post-administration.
Conclusions:
- Cefluprenam (CFLP) demonstrates significant efficacy in treating polymicrobial pyometra.
- A more divided dosing regimen (40 mg/kg q.i.d.) appears more effective than a less frequent regimen (80 mg/kg b.i.d.).
- CFLP is a promising therapeutic agent for polymicrobial infections involving Enterococcus faecalis.
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