Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
High-Performance Liquid Chromatography: Introduction01:11

High-Performance Liquid Chromatography: Introduction

High-performance liquid chromatography(HPLC), formerly referred to as High-pressure liquid chromatography, is a powerful technique used to separate, identify, and quantify components in complex mixtures. The term "high pressure" refers to using high pressure to push the liquid mobile phase through the tightly packed columns.
In HPLC, two phases play a critical role in the separation process:
Capillary Electrophoresis: Applications01:30

Capillary Electrophoresis: Applications

Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
MALDI-TOF Mass Spectrometry01:19

MALDI-TOF Mass Spectrometry

Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...
High-Performance Liquid Chromatography: Types of Detectors01:15

High-Performance Liquid Chromatography: Types of Detectors

The role of the detectors in High-Performance Liquid Chromatography (HPLC) is to analyze the solutes as they exit from the chromatographic column. The detector recognizes the solute's property and generates corresponding electrical signals, which are converted into a readable graph of the detector's response versus elution time called a chromatogram at the computer. There are several types of HPLC detectors, each with its own advantages and limitations, depending on the analyte properties and...
Tandem Mass Spectrometry01:21

Tandem Mass Spectrometry

Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and reduce chemical noise during analyte detection. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Gel Shrinkage in Discontinuous Electrophoresis: How to Stabilize the Electrolyte Boundary in Epitachophoresis - Part 2 - Nongel Solid Support.

ACS omega·2026
Same author

Gel Shrinkage in Discontinuous Electrophoresis: How to Stabilize the Electrolyte Boundary in EpitachophoresisPart 1Gel Selection.

ACS omega·2026
Same author

Editorial for New Frontiers in Electrophoresis.

Talanta·2025
Same author

Correction: Microfluidic device for enhancement and analysis of osteoblast differentiation in three-dimensional cell cultures.

Journal of biological engineering·2025
Same author

3D-printed device for time- and cost-efficient sample preparation and DNA fractionation.

Talanta·2025
Same author

Miniaturized nanoelectrospray interface for coupling capillary electrophoresis with mass spectrometry detection.

Electrophoresis·2024
Same journal

Chromatographic Purification of Complex Natural Products as a Decision Problem: Retention Prediction, Adaptive Optimization, and Experimental Feedback.

Journal of separation science·2026
Same journal

A High-Throughput Analytical Approach Using Polyaniline Doped With Oxalic Acid in Thin Film Solid-Phase Microextraction for the Determination of Personal Care Products in Recreational Waters.

Journal of separation science·2026
Same journal

Simultaneous Determination of Dechlorane-Related Compounds in Fish Muscle by Microwave-Assisted Extraction Combined With Enhanced Matrix Removal for Lipids Cleanup and Gas Chromatography-Tandem Mass Spectrometry.

Journal of separation science·2026
Same journal

Covalent Zwitterionic Peptide-Based Antifouling Coating of the Fused Silica Capillary Applied for CE Separation of Proteins.

Journal of separation science·2026
Same journal

Pharmacokinetic Study of Five Lead Components of Psoraleae Fructus in Human Serum by UHPLC-Q-TOF-MS and UHPLC-QqQ-MS/MS after Oral Administration of Qing'e Pills.

Journal of separation science·2026
Same journal

Passive Blood-Plasma Separation via Constriction-Expansion Geometry in Untreated Paper Microfluidic Devices.

Journal of separation science·2026
See all related articles

Related Experiment Video

Updated: Jul 3, 2026

Ion Exchange Chromatography (IEX) Coupled to Multi-angle Light Scattering (MALS) for Protein Separation and Characterization
10:41

Ion Exchange Chromatography (IEX) Coupled to Multi-angle Light Scattering (MALS) for Protein Separation and Characterization

Published on: April 5, 2019

Multidimensional liquid phase separations for mass spectrometry.

Roman Tomás1, Karel Klepárník, Frantisek Foret

  • 1Institute of Analytical Chemistry, Brno, Czech Republic.

Journal of Separation Science
|July 11, 2008
PubMed
Summary
This summary is machine-generated.

Advanced analytical techniques like genomics and proteomics are crucial for modern research. This review explores 2-D separations, including microfluidics, for analyzing complex biological samples with high resolution.

More Related Videos

Using the GELFREE 8100 Fractionation System for Molecular Weight-Based Fractionation with Liquid Phase Recovery
07:57

Using the GELFREE 8100 Fractionation System for Molecular Weight-Based Fractionation with Liquid Phase Recovery

Published on: December 3, 2009

Identifying Per- and Polyfluorinated Chemical Species with a Combined Targeted and Non-Targeted-Screening High-Resolution Mass Spectrometry Workflow
09:04

Identifying Per- and Polyfluorinated Chemical Species with a Combined Targeted and Non-Targeted-Screening High-Resolution Mass Spectrometry Workflow

Published on: April 18, 2019

Related Experiment Videos

Last Updated: Jul 3, 2026

Ion Exchange Chromatography (IEX) Coupled to Multi-angle Light Scattering (MALS) for Protein Separation and Characterization
10:41

Ion Exchange Chromatography (IEX) Coupled to Multi-angle Light Scattering (MALS) for Protein Separation and Characterization

Published on: April 5, 2019

Using the GELFREE 8100 Fractionation System for Molecular Weight-Based Fractionation with Liquid Phase Recovery
07:57

Using the GELFREE 8100 Fractionation System for Molecular Weight-Based Fractionation with Liquid Phase Recovery

Published on: December 3, 2009

Identifying Per- and Polyfluorinated Chemical Species with a Combined Targeted and Non-Targeted-Screening High-Resolution Mass Spectrometry Workflow
09:04

Identifying Per- and Polyfluorinated Chemical Species with a Combined Targeted and Non-Targeted-Screening High-Resolution Mass Spectrometry Workflow

Published on: April 18, 2019

Area of Science:

  • Biotechnology
  • Analytical Chemistry
  • Molecular Biology

Background:

  • Current research in biology, medicine, and biotechnology relies heavily on analyzing DNA (genomics), proteins (proteomics), and metabolites (metabolomics).
  • Advances in biotechnology necessitate the development of sensitive analytical instrumentation for analyzing minute sample quantities, exemplified by single-cell analysis.
  • Established protocols for complex mixture analysis include 2-D electrophoresis (IEF followed by SDS-PAGE) and chromatography.

Purpose of the Study:

  • To review important aspects of 2-D separation techniques.
  • To highlight advancements in analytical instrumentation for complex biological sample analysis.
  • To discuss the role of microfluidics in high-resolution separation.

Main Methods:

  • Two-dimensional electrophoresis (2-DE), including isoelectric focusing (IEF) and SDS-PAGE.
  • Chromatographic techniques for separating complex mixtures.
  • Mass spectrometry (MS) and Nuclear Magnetic Resonance (NMR) for identification and structure analysis.

Main Results:

  • High-resolution separation of sample components is critical for successful analysis.
  • Current high-resolution proteomic analysis often depends on time-consuming offline methods.
  • Microfluidics offers potential for improved 2-D separation techniques.

Conclusions:

  • 2-D separation techniques, enhanced by microfluidics, are vital for high-resolution analysis in genomics, proteomics, and metabolomics.
  • Further development of analytical instrumentation is needed to meet the demands of analyzing minute biological samples.
  • Integrating advanced separation methods with information-rich techniques like MS and NMR is key for comprehensive biological analysis.