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Comparison of Methods for Isolating Entomopathogenic Fungi from Soil Samples
Published on: January 6, 2022
Bacillus thuringiensis improved isolation methodology from soil samples
M A Santana1, C C Moccia-V, A E Gillis
1Departamento de Biología Celular, Universidad Simón Bolívar, Sartenejas, Baruta, Edo. Miranda, Apartado 89000 Caracas, Venezuela. msantana@usb.ve
Abstract:
Bacillus thuringiensis is a sporulating bacterium, which produces parasporal inclusions toxic to insects. Widely used methodologies to isolate Bt from soil consist of a thermal shock treatment followed by selective germination of spores. The results presented here suggest that a preliminary 5 h dry-heat treatment largely enhance the selectivity of these procedures.
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