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Protocol to Create Chronic Wounds in Diabetic Mice
Published on: September 25, 2019
Wound closure and metabolic parameter variability in a db/db mouse model for diabetic ulcers
Rhonda K Trousdale1, Sharone' Jacobs, David A Simhaee
1Department of OB/GYN, Columbia University Medical Center, New York, New York, USA.
The Journal of Surgical Research
|July 16, 2008
Summary
Diabetic db/db mice show impaired wound healing, but diabetes severity did not correlate with healing rates or variability in this model. Further research is needed to understand diabetic wound healing.
Area of Science:
- Diabetology
- Wound Healing Research
- Animal Models
Background:
- Diabetic foot ulcers are a leading cause of lower extremity amputations.
- The db/db mouse model is frequently used to study diabetes and wound healing.
- Glycemic control is linked to cardiovascular complications in diabetic patients.
Purpose of the Study:
- To investigate the relationship between diabetes severity and impaired wound healing in diabetic db/db mice.
- To explore the variability in wound closure rates among diabetic mice.
Main Methods:
- Excisional wounds were created in adult female C57BLKS/J, db+/-, and db/db mice.
- Wound closure was assessed on multiple postoperative days.
- Fasting blood glucose, insulin, and body weight were measured.
Main Results:
- db/db mice exhibited significantly impaired wound healing by postoperative day 21.
- Wound closure in db/db mice ranged broadly from 24% to 81%.
- No significant correlation was found between wound closure rate and diabetes severity parameters.
Conclusions:
- Diabetic db/db mice demonstrate impaired healing in excisional wound models.
- Variability in wound closure did not correlate with obesity, hyperglycemia, or insulin resistance.
- Basic diabetes parameters do not fully explain wound healing in this mouse model.
