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Published on: June 14, 2020
Differentiated S100A7 expression in infected tonsils and tonsils from allergic individuals
Malin Bryborn1, Anne Månsson, Lars Olaf Cardell
1Laboratory of Clinical and Experimental Allergy Research, Department of Otorhinolaryngology, Malmö University Hospital, Lund University, Malmö, Sweden. malin.bryborn@med.lu.se
FEMS Immunology and Medical Microbiology
|July 16, 2008
Summary
This study found that S100A7 protein expression is reduced in infected and allergic palatine tonsils, suggesting a role in tonsillitis and allergies.
Area of Science:
- Immunology
- Microbiology
Background:
- Palatine tonsils are crucial in immune defense, secreting antimicrobial peptides.
- S100A7 protein exhibits antimicrobial and chemotactic properties.
Purpose of the Study:
- To investigate the impact of inflammation on S100A7 expression in human palatine tonsils.
- To explore the role of S100A7 in recurrent tonsillitis and allergic conditions.
Main Methods:
- Analysis of tonsils from 109 patients (infected/non-infected, allergic/non-allergic).
- Western blot, immunohistochemistry for protein expression, and real-time PCR for mRNA levels.
- In vitro stimulation with lipopolysaccharide.
Main Results:
- S100A7 protein was localized to tonsillar epithelium, B- and T-cell regions, particularly CD8+ T cells.
- Reduced S100A7 mRNA levels were observed in infected and allergic tonsils.
- In vitro lipopolysaccharide stimulation did not alter S100A7 expression.
Conclusions:
- S100A7 expression is modulated by inflammatory and allergic processes in palatine tonsils.
- Findings suggest S100A7 is implicated in recurrent tonsillitis and allergic disease.
