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Updated: Jul 3, 2026

Bilayer Microfluidic Device for Combinatorial Plug Production

Bilayer Microfluidic Device for Combinatorial Plug Production

Published on: December 1, 2023

Microprocessing of liquid plugs for bio/chemical analyses.

Fumihiro Sassa¹, Junji Fukuda, Hiroaki Suzuki

¹Graduate School of Pure and Applied Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8573, Japan.

Analytical Chemistry

|July 17, 2008

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This study presents a microfluidic device for precise liquid plug handling in biochemical analyses. The technology enables controlled sequential reactions and efficient sensing, minimizing cross-contamination for reliable results.

Area of Science:

Biochemistry
Microfluidics
Analytical Chemistry

Background:

Efficient handling of multiple solutions is crucial for complex biochemical analyses.
Microfluidic devices offer miniaturization and precise control for chemical reactions.

Purpose of the Study:

To develop a microfluidic device and operation for efficient liquid plug handling in biochemical analyses.
To enable controlled sequential reactions and rapid mixing within microflow channels.
To demonstrate the device's utility in bio/chemical sensing applications.

Main Methods:

Design and implementation of a T-junction microfluidic device for plug manipulation.
Utilizing unit operations like plug attachment, division, sorting, and formation.
Employing sequential mixing and fluorescence intensity measurements for analysis.

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A Microfluidic Platform for Precision Small-volume Sample Processing and Its Use to Size Separate Biological Particles with an Acoustic Microdevice

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Last Updated: Jul 3, 2026

Bilayer Microfluidic Device for Combinatorial Plug Production

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Published on: December 1, 2023

Fast Enzymatic Processing of Proteins for MS Detection with a Flow-through Microreactor

Fast Enzymatic Processing of Proteins for MS Detection with a Flow-through Microreactor

Published on: April 6, 2016

A Microfluidic Platform for Precision Small-volume Sample Processing and Its Use to Size Separate Biological Particles with an Acoustic Microdevice

A Microfluidic Platform for Precision Small-volume Sample Processing and Its Use to Size Separate Biological Particles with an Acoustic Microdevice

Published on: November 23, 2015

Investigating strategies to minimize cross-contamination using cleansing plugs and low-velocity processing.

Main Results:

Demonstrated controlled sequential reactions and rapid mixing of liquid plugs.
Successfully characterized performance in bio/chemical sensing using L-glutamate oxidase.
Quantified fluorescence intensity from plugs of varying L-glutamate concentrations and pH.
Showcased effective minimization of cross-contamination through optimized flow channel design and processing parameters.

Conclusions:

The developed microfluidic device provides efficient and controlled handling of liquid plugs for biochemical analyses.
The system facilitates sequential reactions, rapid mixing, and simultaneous measurement, enhancing analytical capabilities.
This technology represents a critical component for microprocessing in diverse bio/chemical applications.