Related Experiment Video
Updated: Jul 3, 2026

12:29
mRNA Interactome Capture from Plant Protoplasts
Published on: July 28, 2017
Messenger RNA 3' end formation in plants.
1Department of Plant and Soil Sciences, University of Kentucky, Lexington, KY 40546-0312, USA. aghunt00@uky.edu
Current Topics in Microbiology and Immunology
|July 18, 2008
Summary
Messenger RNA 3' end formation is crucial for mature mRNA in eukaryotes. This review details plant mRNA processing, polyadenylation, and the regulation of key factors involved in gene expression.
Area of Science:
- Molecular Biology
- Plant Science
- Gene Expression
Background:
- Messenger RNA (mRNA) 3' end formation is essential for creating mature, translatable mRNAs in eukaryotes.
- This process involves pre-mRNA cleavage and polyadenylation, adding a poly(A) tail critical for translation initiation and mRNA stability.
- mRNA 3' end formation integrates various mRNA biogenesis steps and is a regulatory point for gene expression.
Purpose of the Study:
- To review the current understanding of mRNA 3' end formation in plants.
- To examine the characteristics of mRNA 3' ends in plant systems.
- To discuss recent insights into the function and regulation of plant polyadenylation factors.
Main Methods:
- Literature review of existing studies on plant mRNA 3' end formation.
- Analysis of research on polyadenylation mechanisms and regulatory factors in plants.
- Synthesis of findings regarding the nature and processing of plant mRNA 3' ends.
Main Results:
- Plant mRNA 3' end formation shares fundamental similarities with other eukaryotes but possesses unique features.
- The poly(A) tail in plants plays vital roles in mRNA stability and translation efficiency.
- Recent studies are elucidating the specific roles and regulatory networks of plant polyadenylation factor subunits.
Conclusions:
- mRNA 3' end formation is a highly regulated process critical for plant gene expression.
- Understanding plant-specific polyadenylation factors is key to comprehending post-transcriptional gene regulation.
- Further research into these factors will illuminate mechanisms controlling plant development and stress responses.
More Related Videos
Related Concept Videos
pre-mRNA Processing
In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a “cap” to the 5’ end of the growing transcript. In this process, a 5’ phosphate is replaced by modified guanosine that has a methyl group attached to it (7-Methyl guanosine). This 5’ cap helps the...
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a “cap” to the 5’ end of the growing transcript. In this process, a 5’ phosphate is replaced by modified guanosine that has a methyl group attached to it (7-Methyl guanosine). This 5’ cap helps the...
Pre-mRNA Processing
In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a “cap” to the 5’ end of the growing transcript. In this process, a 5’ phosphate is replaced by modified guanosine that has a methyl group attached to it (7-Methyl guanosine). This 5’ cap helps the...
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a “cap” to the 5’ end of the growing transcript. In this process, a 5’ phosphate is replaced by modified guanosine that has a methyl group attached to it (7-Methyl guanosine). This 5’ cap helps the...
Pre-mRNA Processing: Modification of pre-mRNA Ends
In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a cap to the 5' end of the growing transcript. In this process, a 5' phosphate is replaced by modified guanosine that has a methyl group attached (7-methyl guanosine). This 5' cap helps the cell...
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a cap to the 5' end of the growing transcript. In this process, a 5' phosphate is replaced by modified guanosine that has a methyl group attached (7-methyl guanosine). This 5' cap helps the cell...
RNA Splicing
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
RNA Splicing
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Riboswitches
Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...

