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A random grid based molecular epidemiological study on EBLV isolates from Germany.

C Freuling¹, N Johnson, D A Marston

¹Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Wusterhausen, Germany. Conrad.Freuling@fli.bund.de

Developments in Biologicals

|July 19, 2008

Summary

This summary is machine-generated.

German bats show stable European Bat Lyssavirus Type 1 (EBLV-1) strains with distinct geographical patterns. Genetic analysis reveals genomic stability and unique insertions in the N-P intergenic region, impacting molecular epidemiology.

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Area of Science:

Veterinary Virology
Molecular Epidemiology
Bat Ecology

Background:

Germany has a high incidence of European Bat Lyssavirus (EBLV) cases in bats.
Previous studies identified German EBLV isolates as EBLV-1 with distinct geographical distributions.

Purpose of the Study:

To investigate the spatial and temporal distribution of EBLV-1 variants in Germany.
To analyze the impact of EBLV-1 variants on molecular epidemiology using phylogenetic analysis.

Main Methods:

Random grid sampling based on GIS was used to select 120 geo-referenced EBLV isolates.
Nucleoprotein (N) and parts of the phosphoprotein (P) genes were sequenced using direct cycle sequencing.
Phylogenetic analysis of the N-gene sequences was performed.

Main Results:

Phylogenetic analysis confirmed high sequence homology among German EBLV-1 isolates.
Identical sequence homologies within specific regions suggest genomic stability and limited geographic spread.
Unique findings include a 6 bp insertion and a single nucleotide insertion in the N-P intergenic region of German EBLV-1 isolates.

Conclusions:

EBLV-1 in Germany exhibits genomic stability with minimal geographic intermixing.
The identified insertions in the N-P intergenic region may be specific to German EBLV-1 strains.
Further research is needed to understand the implications of these genetic variations on virus transmission and epidemiology.