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Field flow fractionation in biomedical analysis.

S Levin1

  • 1Pharmaceutical Chemistry Department, Hebrew University, Jerusalem, Israel.

Biomedical Chromatography : BMC
|May 1, 1991
PubMed
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Field-flow fractionation (FFF) analyzes diverse biomedical samples, from cells to proteins. This review details FFF principles, instrumentation, and techniques, focusing on sedimentation and flow FFF for biomedical applications.

Area of Science:

  • Biochemistry
  • Biophysics
  • Analytical Chemistry

Background:

  • Field-flow fractionation (FFF) is a versatile separation technique.
  • Biomedical analysis requires robust methods for diverse sample types.

Purpose of the Study:

  • To survey biomedical samples analyzed using FFF techniques.
  • To describe the principles, instrumentation, and techniques of FFF.
  • To highlight sedimentation and flow FFF for biomedical applications.

Main Methods:

  • Review of literature on FFF applications in biomedical analysis.
  • Explanation of normal and steric retention modes in FFF.
  • Description of instrumentation and techniques for FFF.

Main Results:

Related Experiment Videos

  • FFF can analyze a wide range of biomedical samples, including whole cells, viruses, nucleic acids, organelles, and proteins.
  • Sedimentation FFF and flow FFF are the primary systems for biomedical applications.
  • Principles of separation and retention modes are illustrated.

Conclusions:

  • FFF is a powerful tool for the characterization of complex biological macromolecules and particles.
  • Sedimentation and flow FFF offer effective solutions for biomedical sample analysis.
  • Further exploration of FFF techniques can advance biomedical research.