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Related Experiment Video

Updated: Jul 3, 2026

A Cell-to-cell Macromolecular Transport Assay in Planta Utilizing Biolistic Bombardment

A Cell-to-cell Macromolecular Transport Assay in Planta Utilizing Biolistic Bombardment

Published on: August 27, 2010

Plant virus-specific transport function. I. Virus genetic control required for systemic spread.

M E Taliansky¹, S I Malyshenko, E S Pshennikova

¹Department of Virology and A. N. Belozersky Laboratory of Molecular Biology and Bioorganic Chemistry, Moscow State University, Moscow 117234, USSR.

|October 30, 1982

Summary

Temperature-sensitive (ts) tobacco mosaic virus (TMV) transport can be complemented by other viruses. This study shows that unrelated viruses, like potato virus X, can facilitate the movement of ts TMV mutants.

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Fluorescence in situ Hybridizations (FISH) for the Localization of Viruses and Endosymbiotic Bacteria in Plant and Insect Tissues

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Published on: February 24, 2014

Area of Science:

Plant Virology
Molecular Plant Pathology

Background:

Tobacco mosaic virus (TMV) exhibits temperature-sensitive (ts) mutations affecting its transport function.
Understanding virus-cell to cell transport mechanisms is crucial for plant disease management.

Purpose of the Study:

To investigate the complementation of a ts TMV mutant (LS1) in its transport function using two model systems.
To determine if temperature-resistant (tr) helper viruses or unrelated viruses can restore the transport of LS1.

Main Methods:

Utilized a host plant system with differential reactions to LS1 and a helper virus at permissive and non-permissive temperatures.
Employed temperature shift treatments (TST) and immunofluorescent microscopy to observe cell-to-cell transport.
Studied virus movement from stem to leaf mesophyll cells in preinfected plants.

Main Results:

Complementation of LS1 transport occurred in mixedly infected plants at non-permissive temperatures, especially after TST.
The ts mutant LS1 could move into and replicate in mesophyll cells preinfected with a tr-helper virus at both 24 and 32 degrees.
Both related tr TMV strains and unrelated potato virus X demonstrated complementation of ts transport.

Conclusions:

Virus transport function can be complemented by both related and unrelated viruses, suggesting a common mechanism or host modification.
Helper viruses may facilitate dependent virus movement by modifying host cells, enabling transport even at non-permissive temperatures.