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Related Concept Videos

Hybridoma Technology01:31

Hybridoma Technology

Hybridoma technology is used for the large-scale production of monoclonal antibodies. Monoclonal antibodies bind to only a single antigenic determinant or epitope. Such antibodies are used in research, diagnostics, and disease therapy. The hybridoma technology established in 1975 by Georges Köhler and Cesar Milstein was awarded the Nobel Prize in Medicine in 1984 for revolutionizing research and therapy.
Hybridoma Selection
Commonly used fusion techniques — electroporation, polyethylene glycol...
Cell-mediated Immune Responses01:40

Cell-mediated Immune Responses

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Antibody Structure01:10

Antibody Structure

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Antibodies, also known as immunoglobulins (Ig), are essential players of the adaptive immune system. These antigen-binding proteins are produced by B cells and make up 20 percent of the total blood plasma by weight. In mammals, antibodies fall into five different classes, which each elicits a different biological response upon antigen binding.
The Y-Shaped Structure of Antibodies Consists of Four Polypeptide Chains
Antibodies consist of four polypeptide chains: two identical heavy...
Special Features of Adaptive Immunity01:20

Special Features of Adaptive Immunity

The adaptive immune system, a crucial component of the overall immune response, offers a highly specialized defense against pathogens. It involves specific cell types and features, enabling it to combat infections effectively and efficiently.
The primary cell types involved in adaptive immunity are T cells and B cells. Each type has a unique role in defending the body against pathogens. T cells are responsible for cell-mediated immunity. They identify and eliminate infected cells directly,...
Cells of the Adaptive Immune Response01:23

Cells of the Adaptive Immune Response

The T and B lymphocytes of the adaptive immune system develop from common lymphoid progenitor cells in the bone marrow. These progenitors give rise to precursors that eventually develop into both T and B lymphocytes. As these precursors mature, they gain the ability to detect and respond to foreign antigens in the body, a process known as immunocompetence. Additionally, these precursors acquire self-tolerance, a process that ensures they do not react to self-antigens. This intricate system...

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Related Experiment Video

Updated: Jul 3, 2026

Histological Quantification to Determine Lung Fungal Burden in Experimental Aspergillosis
09:52

Histological Quantification to Determine Lung Fungal Burden in Experimental Aspergillosis

Published on: March 9, 2018

Cultivated anti-Aspergillus T(H)1 cells.

Thomas Lehrnbecher1, Olaf Beck, Ulrike Koehl

  • 1Pediatric Hematology and Oncology, Children's Hospital III, Johann Wolfgang Goethe University, Frankfurt, Germany. Thomas.Lehrnbecher@kgu.de

Medical Mycology
|July 25, 2008
PubMed
Summary

Researchers isolated and grew Aspergillus-specific T cells to combat invasive fungal infections after stem cell transplants. These T cells show potential for adoptive immunotherapy, though further research is needed to identify optimal patient candidates.

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Detection of Invasive Pulmonary Aspergillosis in Haematological Malignancy Patients by using Lateral-flow Technology
08:01

Detection of Invasive Pulmonary Aspergillosis in Haematological Malignancy Patients by using Lateral-flow Technology

Published on: March 22, 2012

Area of Science:

  • Immunology
  • Transplantation Medicine
  • Mycology

Background:

  • Invasive aspergillosis is a significant risk for allogeneic stem cell transplant recipients.
  • Lymphocytes play a crucial role in controlling fungal infections.
  • Adoptive transfer of anti-fungal T cells could restore immune defenses.

Purpose of the Study:

  • To isolate and characterize human T cells with activity against Aspergillus fumigatus.
  • To assess the potential of these T cells for adoptive immunotherapy in transplant patients.

Main Methods:

  • Human T cells were stimulated with Aspergillus fumigatus extract.
  • Interferon-gamma (IFN-γ) secretion assay was used for isolation.
  • Cells were cultured for 14 days and characterized as T(H)1 cells.
  • Proliferation, antigen specificity, and anti-fungal activity were assessed.
  • Clinical-scale generation methods were established.

Main Results:

  • Activated T cells were identified as T(H)1 cells with proliferative capacity.
  • Generated T cells responded to Aspergillus flavus, Aspergillus niger, and Penicillium chrysogenum antigens.
  • T cells demonstrated efficacy against Aspergillus hyphae and reduced alloreactivity.
  • Clinical-scale generation of anti-Aspergillus T cells was achieved.

Conclusions:

  • Adoptive immunotherapy using anti-Aspergillus T cells is a potential strategy for invasive aspergillosis.
  • Further research is required to determine patient populations that would benefit from this therapy.
  • Clinical trials need to address open questions regarding patient selection and efficacy.