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Related Experiment Video

Updated: Jul 3, 2026

Microfluidic Chips Controlled with Elastomeric Microvalve Arrays
18:11

Microfluidic Chips Controlled with Elastomeric Microvalve Arrays

Published on: October 1, 2007

A high-yield method for generating mass-transfer gradients in elastomer microfluidics using impermeable capillaries.

Mike Pinelis1, Leonid Shamban2, Andreja Jovic3

  • 1Department of Electrical Engineering and Computer Science, University of Michigan, Ann Arbor, USA. mikepinelis@earthlink.net.

Biomedical Microdevices
|July 26, 2008
PubMed
Summary

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We developed a low-cost, high-throughput method for creating gas gradient devices using elastomer microfluidics. This technique enables rapid, replicate experiments to study cell responses to various chemical gradients.

Area of Science:

  • Biomedical Engineering
  • Microfluidics
  • Cell Biology

Background:

  • Generating precise chemical gradients is crucial for understanding cell behavior.
  • Traditional methods for gradient generation can be complex and low-throughput.
  • Elastomer microfluidics offers a versatile platform for biological experimentation.

Purpose of the Study:

  • To develop a robust, inexpensive, and high-throughput method for fabricating gas gradient generation devices.
  • To demonstrate the integration of these devices with microfluidic systems.
  • To characterize chemical gradients and observe cell responses.

Main Methods:

  • Combined classic mass-transfer limited techniques with elastomer microfluidics.
  • Fabricated and assembled dozens of replicate devices per day.

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A Gradient-generating Microfluidic Device for Cell Biology
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A Gradient-generating Microfluidic Device for Cell Biology

Published on: August 30, 2007

Related Experiment Videos

Last Updated: Jul 3, 2026

Microfluidic Chips Controlled with Elastomeric Microvalve Arrays
18:11

Microfluidic Chips Controlled with Elastomeric Microvalve Arrays

Published on: October 1, 2007

Fabricating High-viscosity Droplets using Microfluidic Capillary Device with Phase-inversion Co-flow Structure
08:02

Fabricating High-viscosity Droplets using Microfluidic Capillary Device with Phase-inversion Co-flow Structure

Published on: April 17, 2018

A Gradient-generating Microfluidic Device for Cell Biology
11:05

A Gradient-generating Microfluidic Device for Cell Biology

Published on: August 30, 2007

  • Interfaced devices with microfluidics to control gradient parameters.
  • Applied the method to characterize pH and cell viability gradients.
  • Observed differentiating myoblasts in an oxygen gradient.

    • Main Results:

    • Successfully fabricated high-throughput gas gradient generation devices.
    • Demonstrated the ability to vary gradient parameters and interface with microfluidics.
    • Characterized pH and cell viability gradients in mass-transfer limited cultures.
    • Observed cellular morphology changes in response to an oxygen gradient.

    Conclusions:

    • The developed method provides a cost-effective and efficient approach for generating gas gradients.
    • This technique facilitates high-throughput studies of cell responses to chemical gradients.
    • The devices are versatile and applicable to various biological research areas, including cell differentiation and viability studies.