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Related Experiment Video

Updated: Jul 3, 2026

"Cell Surface Capture" Workflow for Label-Free Quantification of the Cell Surface Proteome
06:31

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Published on: March 24, 2023

Cell surface proteomics identifies molecules functionally linked to tumor cell intravasation.

Erin M Conn1, Mark A Madsen, Benjamin F Cravatt

  • 1Departments of Cell Biology, The Scripps Research Institute, La Jolla, California 92037, USA.

The Journal of Biological Chemistry
|July 29, 2008
PubMed
Summary

Researchers identified key cell surface proteins, including tissue factor (TF), that drive tumor cell intravasation and metastasis. Inhibiting TF significantly reduced cancer cell spread, highlighting its role in early dissemination. This study offers insights into molecular mechanisms of cancer spread.

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Assessing Tumor Microenvironment of Metastasis Doorway-Mediated Vascular Permeability Associated with Cancer Cell Dissemination using Intravital Imaging and Fixed Tissue Analysis

Published on: June 26, 2019

Area of Science:

  • Oncology
  • Proteomics
  • Cell Biology

Background:

  • Tumor cell intravasation and dissemination are critical steps in metastasis.
  • Understanding the molecular determinants of these processes is essential for developing targeted therapies.

Purpose of the Study:

  • To identify and characterize cell surface molecules that differ between high and low intravasating fibrosarcoma variants.
  • To functionally validate the role of identified molecules in tumor cell dissemination.

Main Methods:

  • Generation of congenic HT-1080 fibrosarcoma variants with distinct intravasation capacities.
  • Comparative cell surface proteomic analysis using biotinylation, avidin precipitation, and tandem mass spectrometry.
  • Biochemical validation (Western blot) and in vivo functional studies (chick embryo model, siRNA, antibody inhibition).

Main Results:

  • 47 differentially expressed cell surface molecules were identified between HT-1080 variants.
  • TIMP-2, NCAM-1, JAM-C, and tissue factor (TF) were validated as differentially expressed.
  • Inhibition of tissue factor (TF) significantly reduced tumor cell intravasation in vitro and in vivo.

Conclusions:

  • Cell surface proteomic analysis is effective for identifying molecules involved in tumor cell intravasation.
  • Tissue factor (TF) plays a crucial role in the early stages of tumor cell dissemination and metastasis.
  • Targeting TF may represent a therapeutic strategy to inhibit cancer metastasis.