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Protein Dynamics in Living Cells

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...

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Related Experiment Video

Updated: Jul 16, 2025

High-resolution Spatiotemporal Analysis of Receptor Dynamics by Single-molecule Fluorescence Microscopy

High-resolution Spatiotemporal Analysis of Receptor Dynamics by Single-molecule Fluorescence Microscopy

Published on: July 25, 2014

Deciphering Drug Targets and Actions with Single-Cell and Spatial Resolution.

Zhengyuan Pang¹, Benjamin F Cravatt², Li Ye^1,3

¹Department of Neuroscience, The Scripps Research Institute, La Jolla, California, USA;

Annual Review of Pharmacology and Toxicology

|September 18, 2023

Summary

This summary is machine-generated.

New methods link drug-target interactions with cellular and spatial tissue heterogeneity. Integrating single-cell and spatial omics with drug imaging will advance understanding of in vivo drug mechanisms.

Keywords:

CATCH chemoproteomics drug-target interactions mechanism of action single cell spatial omics

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Last Updated: Jul 16, 2025

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Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection

Published on: July 6, 2022

Area of Science:

Chemical biology
Genomics
Molecular biology
Biotechnology

Background:

Advances in chemical, molecular, and genetic methods enable whole-proteome and genome drug-target interaction identification.
Single-cell and spatial omics technologies are transforming the understanding of biological systems' molecular architecture.
A gap exists in aligning traditional drug action understanding (molecular affinities) with in vivo cellular and spatial tissue heterogeneity.

Purpose of the Study:

To review state-of-the-art methods for profiling drug-target interactions.
To discuss emerging multiomics tools for delineating tissue heterogeneity at single-cell resolution.
To highlight advances in in situ small-molecule drug imaging.

Main Methods:

Review of current drug-target interaction profiling techniques.
Exploration of single-cell and spatial omics for tissue heterogeneity analysis.
Focus on clearing-assisted tissue click chemistry (CATCH) for multiplexable in situ drug imaging.

Main Results:

Identification of methods to profile drug-target interactions across proteome and genome.
Characterization of tissue heterogeneity using single-cell and spatial omics.
Demonstration of high-resolution, multiplexable in situ small-molecule drug imaging via CATCH.

Conclusions:

Integration of single-cell and spatial omics platforms is crucial for future drug discovery.
Bridging the gap between molecular affinities and in vivo heterogeneity is key to understanding drug mechanisms.
The future framework for defining in vivo drug-target interactions will incorporate multiomics data and advanced imaging techniques.