Enhancement of vascular function mediated by insulin and insulin-like growth factor-1 following single exercise...

Exercise is well-known in improving vascular functions, but the underlying mechanism has not been totally understood. The aim of this study was to examine whether single exercise session acutely enhances insulin-induced and insulin-like growth factor-1 (IGF-1)-induced vasorelaxation. Twenty-four male Wistar rats at age of 12 weeks were randomly divided into two groups, control (n = 12) and exercise (n = 12) group. The exercise group ran on a treadmill at a speed of 18 m/min for 60 min. Immediately after exercise, insulin-induced and IGF-1-induced vasorelaxant responses were evaluated by the isometric tension of aortic rings in the organ baths. The roles of phosphatidylinositol 3-kinase (PI3K) and nitric oxide synthase (NOS) in vasorelaxant responses were examined by treating selective inhibitors, such as wortmannin (an inhibitor of PI3K) and N(omega)-nitro-L-arginine methyl ester (L-NAME, a NOS inhibitor). In addition, the vascular responses to sodium nitroprusside (SNP), a NO donor, were examined. We found that single exercise session significantly enhanced vasorelaxation mediated by insulin and IGF-1 in rat aortas (P < 0.01). Also, the exercise-enhanced vasorelaxation was abolished by wortmannin or L-NAME. There was no significant difference of SNP-induced vasorelaxation between control and exercise groups. These results indicate that single exercise session acutely enhances insulin-induced and IGF-1-induced vasorelaxation through the PI3K-NOS-dependent pathway.