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Updated: Jul 3, 2026

Computed Tomography-guided Time-domain Diffuse Fluorescence Tomography in Small Animals for Localization of Cancer Biomarkers

Computed Tomography-guided Time-domain Diffuse Fluorescence Tomography in Small Animals for Localization of Cancer Biomarkers

Published on: July 17, 2012

Fluorescent optical tomography with large data sets.

George Y Panasyuk¹, Zheng-Min Wang, John C Schotland

¹Department of Bioengineering, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA.

|August 2, 2008

Summary

This summary is machine-generated.

Fast algorithms for optical tomography (OT) can now reconstruct fluorescent contrast agent concentrations. This advancement enhances imaging of highly scattering biological samples using CCD-based devices.

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Area of Science:

Biomedical Optics
Medical Imaging
Computational Imaging

Background:

Optical tomography (OT) uses CCD-based imaging for highly scattering biological samples.
Large datasets (up to 10^9 measurements) require fast reconstruction algorithms.
Previous work focused on imaging intrinsic absorption coefficients.

Purpose of the Study:

To modify existing fast algorithms for optical tomography.
To enable reconstruction of fluorescent contrast agent concentrations.
To enhance the capabilities of OT for biological sample analysis.

Main Methods:

Development and experimental application of fast algorithms for optical tomography.
Modification of previously developed algorithms.
Reconstruction of fluorescent contrast agent concentrations from large datasets.

Main Results:

Demonstrated successful modification of OT algorithms.
Successfully reconstructed fluorescent contrast agent concentrations.
Validated the enhanced capability for imaging biological samples.

Conclusions:

Modified fast algorithms are effective for reconstructing fluorescent contrast agent concentrations in OT.
This advancement significantly enhances the potential of optical tomography for biological imaging.
The technique is applicable to highly scattering media using CCD-based imaging devices.