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Related Experiment Video

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Ultrasonic-augmented Primary Adult Fibroblast Isolation

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Intestinal smooth muscle cell maintenance by basic fibroblast growth factor.

Min Lee¹, Benjamin M Wu, Matthias Stelzner

¹Department of Bioengineering, University of California, Los Angeles, California 90095, USA.

Tissue Engineering. Part A

|August 6, 2008

Summary

Basic fibroblast growth factor (bFGF) enhances intestinal tissue regeneration. Microsphere delivery of bFGF to scaffolds significantly improved smooth muscle cell survival and blood vessel formation, crucial for functional intestinal repair.

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Area of Science:

Regenerative Medicine
Biomaterials Science
Gastroenterology

Background:

Short bowel syndrome necessitates novel therapeutic strategies.
Intestinal tissue engineering aims to restore gut function via functional smooth muscle regeneration.
Smooth muscle proliferation and angiogenesis are critical for functional intestinal repair.

Purpose of the Study:

To investigate local basic fibroblast growth factor (bFGF) delivery methods for enhancing intestinal smooth muscle tissue engineering.
To evaluate the efficacy of collagen coating versus microsphere encapsulation for bFGF delivery.
To assess the impact of bFGF on smooth muscle cell proliferation, survival, and angiogenesis in engineered constructs.

Main Methods:

Two bFGF delivery methods were tested: incorporation into collagen coating and encapsulation into poly(D,L-lactic-co-glycolic acid) microspheres.
Cell-seeded scaffolds were implanted into the omentum and retrieved at 4, 14, and 28 days.
Cell density, blood vessel formation, and alpha-smooth muscle actin expression were quantified.

Main Results:

Significant increases in cell density and vascularization were observed at 28 days with 10 microg bFGF in collagen coating compared to controls.
Microsphere encapsulation of 1 microg bFGF yielded results comparable to 10 microg bFGF in collagen, outperforming collagen incorporation of 1 microg bFGF.
Implanted cells predominantly expressed alpha-smooth muscle actin, indicating smooth muscle differentiation.

Conclusions:

Local delivery of bFGF is crucial for promoting cell proliferation and angiogenesis in intestinal tissue engineering.
Poly(D,L-lactic-co-glycolic acid) microsphere encapsulation offers an effective strategy for bFGF delivery.
Engineered scaffolds with microsphere-encapsulated bFGF show promise for regenerating functional intestinal smooth muscle.