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06:21
Imaging of Biological Tissues by Desorption Electrospray Ionization Mass Spectrometry
Published on: July 12, 2013
Transmission mode desorption electrospray ionization
Joseph E Chipuk1, Jennifer S Brodbelt
1Department of Chemistry and Biochemistry, University of Texas at Austin, Austin, Texas 78712, USA.
Journal of the American Society for Mass Spectrometry
|August 8, 2008
Summary
A novel transmission mode desorption electrospray ionization (TM-DESI) simplifies liquid sample analysis. This method enables rapid, low-volume analysis without extensive sample preparation, reducing time and effort for high-throughput screening.
Area of Science:
- Analytical Chemistry
- Mass Spectrometry
Background:
- Traditional desorption electrospray ionization (DESI) requires specific spray angles and distances.
- Sample preparation for DESI often involves solvent evaporation, adding time and complexity.
Purpose of the Study:
- To introduce a new transmission mode for DESI (TM-DESI) to streamline liquid sample analysis.
- To demonstrate the efficiency and applicability of TM-DESI for rapid screening.
Main Methods:
- Developed a TM-DESI configuration where the electrospray is transmitted through a sampling mesh.
- Analyzed liquid samples and extracts using microliters of sample with minimal optimization.
- Evaluated key figures of merit for model compounds using the TM-DESI technique.
Main Results:
- TM-DESI allows rapid analysis of liquid samples and residues in seconds.
- The technique eliminates the need for rigorous optimization of spray parameters and solvent evaporation.
- Demonstrated successful application in high-throughput screening of liquid mixtures.
Conclusions:
- TM-DESI offers a simplified and faster approach for analyzing liquid samples and extracts.
- This method is suitable for high-throughput screening applications in various matrices.
- TM-DESI significantly reduces sample preparation time and analytical workload.
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