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Mechanical Separation and Protein Solubilization of the Outer and Inner Perivitelline Sublayers from Hen's Eggs
Published on: January 27, 2021
Structural and physicochemical characteristics of novel basic proteins isolated from duck egg white
Supaporn Naknukool1, Shigeru Hayakawa, Yuanxia Sun
1Department of Applied Biological Science, Faculty of Agriculture, Kagawa University, Miki, Japan.
Bioscience, Biotechnology, and Biochemistry
|August 8, 2008
Summary
Novel duck basic proteins (dBPS(1) and dBPS(2)) were identified from egg white. These heat-stable proteins share ancestry with related avian species and are found in the oviduct and gallbladder.
Area of Science:
- Biochemistry
- Proteomics
- Avian Biology
Background:
- Egg white proteins are a rich source of novel bioactive compounds.
- Basic proteins play diverse roles in biological systems.
- Understanding avian protein diversity is crucial for comparative biology.
Purpose of the Study:
- To isolate and characterize novel basic proteins from duck egg white.
- To investigate the structural, physicochemical, and phylogenetic properties of these proteins.
- To determine the distribution of these proteins within the duck.
Main Methods:
- Cation-exchange and gel filtration chromatography for protein isolation.
- Protein sequencing, MALDI-TOF mass spectrometry, and isoelectric focusing for characterization.
- Fourier Transform Infrared (FT-IR) spectroscopy and indirect ELISA for structural and distribution analysis.
Main Results:
- Two novel basic proteins, duck basic protein small 1 (dBPS(1)) and 2 (dBPS(2)), were isolated.
- Both proteins consist of 39 amino acid residues, feature three disulfide bonds, and exhibit high molecular masses (4,373 and 4,486 Da).
- dBPS(2) showed sequence homology with cygnin and meleagrin, suggesting shared ancestry with other avian species; both proteins demonstrated high heat stability (Td > 98°C) and were detected in the oviduct and gallbladder.
Conclusions:
- dBPS(1) and dBPS(2) are novel, heat-stable avian basic proteins.
- Phylogenetic analysis suggests a common evolutionary origin with related proteins from other bird species.
- The presence of these proteins in the oviduct and gallbladder indicates potential physiological roles in ducks.
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