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[Promoter activity of B-cell maturation antigen gene in mice]
Shao-Li Deng1, Xiao-Xing Cheng, Rui Jian
1Department of Medical Lab. Daping Hospital, Third Military Medical University, Chongqing 400038, China. dengl072@yahoo.com.cn
Aim:
To investigate the promoter activity of BCMA gene 5'-flanking regionfor the study the regulation mechanism of BCMA expression.
Methods:
Luciferase reporter plasmids containing 5'-flanking region of BCMA gene and serial deletions of the fragment were constructed. The luciferase expression was observed after these reporters were transfected into J558L, 293T and Hela cells.
Results:
The highest transcriptional activation was expressed in pGL3-B157 reporter, followed by pGL3-B607, pGL3-B359, pGL3-B93, and pGL3-B820 in sequence. The highest transcriptional activation was in J558L cell.
Conclusion:
The 5'-flanking sequence from -820 to +145 bp of BCMA is of promoter activity. Serial deletion analysis of the promoter region of BCMA gene suggests the sequence from -93 to +145 could be a core promoter region.
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